請用此 Handle URI 來引用此文件: http://hdl.handle.net/11455/71038
標題: Transgenic Acacia sinuata from Agrobacterium tumefaciens-mediated transformation of hypocotyls
作者: Vengadesan, G.
Amutha, S.
Muruganantham, M.
Anand, R.P.
Ganapathi, A.
關鍵字: Agrobacterium transformation
acetosyringone
GUS
leguminous tree
microcuttings
phosphinothricin
genetic-transformation
robinia-pseudoacacia
black locust
regeneration
tree
plants
biotechnology
expression
herbicide
cultures
期刊/報告no:: Plant Cell Reports, Volume 25, Issue 11, Page(s) 1174-1180.
摘要: Transgenic herbicide tolerant Acacia sinuata plants were produced by transformation with the bar gene conferring phosphinothricin resistance. Precultured hypocotyl explants were infected with Agrobacterium tumefaciens strain EHA105 in the presence of 100 mu M acetosyringone and shoots regenerated on MS (Murashige and Skoog, 1962, Physiol Plant 15:473-497) medium with 13.3 mu M benzylaminopurine, 2.6 mu M indole-3-acetic acid, 1 g l(-1) activated charcoal, 1.5 mg l(-1) phosphinothricin, and 300 mg l(-1) cefotaxime. Phosphinothricin at 1.5 mg l(-1) was used for the selection. Shoots surviving selection on medium with phosphinothricin expressed GUS. Following Southern hybridization, eight independent shoots regenerated of 500 cocultivated explants were demonstrated to be transgenic, which represented transformation frequency of 1.6%. The transgenics carried one to four copies of the transgene. Transgenic shoots were propagated as microcuttings in MS medium with 6.6 mu M 6-benzylaminopurine and 1.5 mg l(-1) phosphinothricin. Shoots elongated and rooted in MS medium with gibberellic acid and indole-3-butyric acid, respectively both supplemented with 1.5 mg l(-1) phosphinothricin. Micropropagation of transgenic plants by microcuttings proved to be a simple means to bulk up the material. Several transgenic plants were found to be resistant to leaf painting with the herbicide Basta.
URI: http://hdl.handle.net/11455/71038
ISSN: 0721-7714
文章連結: http://dx.doi.org/10.1007/s00299-006-0176-8
顯示於類別:期刊論文

文件中的檔案:
沒有與此文件相關的檔案。


在 DSpace 系統中的文件,除了特別指名其著作權條款之外,均受到著作權保護,並且保留所有的權利。