Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/71041
標題: Efficient rooting for establishment of papaya plantlets by micropropagation
作者: Yu, T.A.
Yeh, S.D.
Cheng, Y.H.
Yang, J.S.
關鍵字: aeration
agar
papaya rooting
plantlet survival
vermiculite
carica-papaya
l invitro
growth
regeneration
propagation
explants
culture
shoots
期刊/報告no:: Plant Cell Tissue and Organ Culture, Volume 61, Issue 1, Page(s) 29-35.
摘要: A low cost micropropagation protocol to produce high quality root systems which are easy and economical to acclimatize is essential for large-scale micropropagation of papaya (Carica papaya L.). In this study, individual shoots (> 0.5 cm) with 2 similar to 3 leaves from in vitro papaya multiple shoots were cultured on MS agar medium containing 2.5 mu M IBA under dark conditions for 1 week for root induction. They were then transferred to agar or vermiculite media, containing half strength MS medium, under aerated or non-aerated conditions, for root development. Rooting percentage of shoots cultured for 2 weeks in aerated vermiculite was 94.5%, compared with 90.0% in non-aerated vermiculite, 71.1% in aerated agar, and 62.2% in non-aerated agar. Shoots with roots were acclimated in vermiculite under 100% RH for 1 week and then under ambient conditions for 2 weeks in a temperature-controlled growth chamber (28 degrees C). The survival rates of the plantlets were 94.5% from aerated vermiculite, 87.8% from non-aerated vermiculite, 42.2% from aerated agar, and 35.6% from non-aerated agar. Thus, root induction in low-concentration IBA agar medium followed by root development in vermiculite containing half strength MS medium under aerated conditions results in efficient rooting of in vitro papaya shoots.
URI: http://hdl.handle.net/11455/71041
ISSN: 0167-6857
文章連結: http://dx.doi.org/10.1023/a:1006475901439
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