Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/71042
標題: Enhancement of in vitro growth of papaya multishoots by aeration
作者: Lai, C.C.
Yu, T.A.
Yeh, S.D.
Yang, J.S.
關鍵字: ethylene
micropropagation
oxygen
tissue culture
plant-regeneration
carbon-dioxide
ethylene
cultures
transformation
petioles
invitro
期刊/報告no:: Plant Cell Tissue and Organ Culture, Volume 53, Issue 3, Page(s) 221-225.
摘要: Efficient micropropagation of papaya (Carica papaya L.) has become crucial for multiplication of specific sex types of papaya or transgenic lines resistant to virus infection. In this study, aeration at different intervals with a 0.02 mu m filter disc in the closure of culture flasks ensured exchange of gas components. The effect of aeration on development of multibuds to multishoots was investigated. Multibuds grown, in culture flasks after one-week without aeration followed by a two-week aeration treatment caused a 41% increase in the number of shoots greater than or equal to 0.5 cm, 42% increase in leaf expansion, and 17% increase in leaf numbers in comparison with unaerated materials. Ethylene and oxygen concentrations in the culture flasks were measured by gas chromatography and oxygen electrode at weekly intervals during the culture period. Oxygen concentrations were slightly different between aerated and unaerated culture flasks. Ethylene in the unaerated flask reached the highest level (0.11 ppm) 2 weeks after the treatment, while accumulation of ethylene in the aerated flasks was not detected. The multishoots grown for 3 weeks without aeration showed growth retardation on leaves and epinasty on petioles.
URI: http://hdl.handle.net/11455/71042
ISSN: 0167-6857
文章連結: http://dx.doi.org/10.1023/a:1006004216935
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