Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/71289
標題: DELAY OF LPS-INDUCED ACUTE LUNG INJURY RESOLUTION BY SOLUBLE IMMUNE COMPLEXES IS NEUTROPHIL DEPENDENT
作者: Wu, C.L.
Lin, L.Y.
Yeh, H.M.
Chan, M.C.
Yang, C.H.
Hsueh, C.M.
關鍵字: Acute lung injury
immune complexes
neutrophil
apoptosis
Fc gamma R
respiratory-distress-syndrome
igg receptors
cross-linking
u937 cells
apoptosis
involvement
activation
mechanisms
assays
model
期刊/報告no:: Shock, Volume 32, Issue 3, Page(s) 276-285.
摘要: The pathophysiological role of soluble immune complexes (SICXs) and its relationship with neutrophils were investigated in LPS-induced acute lung injury (ALI) animal model (Sprague-Dawley rat) and through the in vitro studies. Results showed that LPS-induced SICX was timely related to changes of tumor necrosis factor alpha and macrophage inflammatory protein 2 (inflammatory cytokines) in bronchoalveolar lavage fluid. In vitro study showed that SICX can bind to Fc gamma R (CD64 and CD32 or CD16) to prevent the apoptosis of neutrophils. The SICX-mediated apoptosis inhibition was extracellular signal-regulated kinase (ERK) or phosphoinositide 3 kinase dependent and was interrupted by PD98059 and LY294002. In vivo, additional amount of SICX exacerbated the lung injury caused by LPS. LPS-induced lung injury and macrophage inflammatory protein 2 release, however, were prevented by CD64 and CD32 blockers (decoy antibodies). In conclusion, excessive amount of SICX in lung can act through Fc gamma Rs to protect bronchoalveolar lavage fluid neutrophils from apoptosis that eventually lead to delayed resolution of ALI caused by LPS. Blockade of SICX engagement of CD32 and CD64 (with decoy antibodies) could interrupt SICX-mediated protection of neutrophils and protect lung from LPS-induced injury. The decoy antibodies may therefore have therapeutic utility in ALI.
URI: http://hdl.handle.net/11455/71289
ISSN: 1073-2322
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