請用此 Handle URI 來引用此文件: http://hdl.handle.net/11455/71577
標題: DNA-binding property of recombinant capsid protein of Japanese encephalitis virus
作者: Tseng, H.N.
Lee, C.C.
Wong, N.L.
Chen, S.O.
Liu, J.J.
關鍵字: Japanese encephalitis virus
capsid protein
electrophoresis mobility
shift assay
core protein
nuclear-localization
dengue virus
flavivirus kunjin
envelope protein
yl-strain
rna
expression
genome
replication
期刊/報告no:: Virus Genes, Volume 35, Issue 3, Page(s) 483-488.
摘要: Japanese encephalitis virus (JEV) is a member of the Flaviviridae family, and it is capable of inducing febrile syndrome, encephalitis and death. In this study, the cDNA of JEV-YL capsid (core) protein were cloned and expressed in E. coli. Three expressed recombinant clones (pET32/CORE, pET32/CD and pET32/C2D) including different parts of capsid protein, were constructed from JEV cDNA clone, pJE-S. These recombinant proteins (34, 31, and 26 kDa, respectively) containing an amino terminal tag of six histidines were isolated by the nickel chelate affinity chromatography and the purified products were identified by Western blotting with anti-serum of JEV-infected swine. To examine DNA binding property of the capsid protein, the purified recombinant proteins were assayed by electrophoretic mobility shift assay. The results showed the capsid proteins had the abilities to bind DNA, except the prominent product of pET32/C2D, which had deletion in the middle hydrophobic region of capsid protein and revealed the coding region of the amino acid, F-46 to P-61, is mediating the DNA binding ability. This study suggests a possible regulatory role for capsid protein in the pathway of JEV infection.
URI: http://hdl.handle.net/11455/71577
ISSN: 0920-8569
文章連結: http://dx.doi.org/10.1007/s11262-006-0075-7
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