請用此 Handle URI 來引用此文件: http://hdl.handle.net/11455/71595
標題: Protein nitration is associated with increased proteolysis in skeletal muscle of bile duct ligation-induced cirrhotic rats
作者: Wang, Y.Y.
毛嘉洪
Lin, S.Y.
Chuang, Y.H.
Mao, C.H.
Tung, K.C.
Sheu, W.H.H.
董光中
關鍵字: ubiquitin-proteasome pathway
nitric-oxide synthase
chronic
heart-failure
oxidative stress
degradation
activation
disease
peroxynitrite
malnutrition
inhibitors
期刊/報告no:: Metabolism-Clinical and Experimental, Volume 59, Issue 4, Page(s) 468-472.
摘要: Cirrhosis is characterized by skeletal muscle wasting. In this study, the effects of nitric oxide production on skeletal muscle protein nitration and degradation in cirrhosis were investigated. Cirrhosis was induced by bile duct ligation (BDL) in Sprague-Dawley rats for 4 weeks. The BDL-induced cirrhotic rats and sham-operated rats were then injected daily with either saline or N(G)-L-nitro-arginine methyl ester (L-NAME) for 7 days from week 4 to week 5, after which nitrite/nitrate, glutathione reduction, as well as protein nitration, ubiquitination, and degradation were assessed in skeletal muscle. Elevated muscular nitrite/nitrate concentrations, protein nitration, total ubiquitin conjugates, and degradation fragments of myosin heavy chain as well as diminished glutathione reduction levels were observed in BDL-induced cirrhotic rats as compared with controls. Administration of L-NAME for 1 week led to reduction of nitrite/nitrate levels; protein nitration was also decreased in the skeletal muscle. In addition, ubiquitination of muscular proteins and degradation of myosin heavy chain were significantly diminished after treatment of L-NAME. In conclusion, nitrosative stress occurred in the skeletal muscle of BDL-induced cirrhotic rats and may lead to increased proteolysis of muscle-specific structural proteins. (C) 2010 Elsevier Inc. All rights reserved.
URI: http://hdl.handle.net/11455/71595
ISSN: 0026-0495
文章連結: http://dx.doi.org/10.1016/j.metabol.2009.07.035
顯示於類別:獸醫學院

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