請用此 Handle URI 來引用此文件: http://hdl.handle.net/11455/71598
標題: Antibacterial activity of short hydrophobic and basic-rich peptides
作者: Chen, P.W.
毛嘉洪
Shyu, C.L.
Mao, F.C.
關鍵字: 15-residue lactoferricin derivatives
n-terminal region
antimicrobial
peptide
bovine lactoferrin
tryptophan-rich
binding
protein
期刊/報告no:: American Journal of Veterinary Research, Volume 64, Issue 9, Page(s) 1088-1092.
摘要: Objective-To design short and potent analogs of bovine lactoferricin by use of the concepts of lipophilic bulk and cationic charge. Sample Population-5 synthetic peptides of bovine lactoferricin. Procedure-Antibacterial peptides were constructed by synthesizing several decapeptides rich in arginine and tryptophan. Basic residues of bovine lactoferricin (bLf 20-29; residues 20 to 29) were modified by substitution with arginine or lysine and nonbasic residues were modified by substitution with tryptophan, phenylalanine, or isoleucine. Synthetic peptides of bovine lactoferrin (LFB) were designated as LFB-RW (RRWWWRWRRW), LFB-KW (KKWWWKWKKW), LFB-RWa (RRWWRRWRRW), LFB-RF (RRFFFRFRRF), and LFB-RI (RRIIIRWRRI), where R, K, W, F, and I stand for arginine, lysine, tryptophan, phenylalanine, and isoleucine, respectively. Peptides were evaluated by determining their minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) against Escherichia coli, Staphylococcus aureus, and Enterococcus faecalis. Results-LFB-RW, LFB-KW, and LFB-RWa possessed equivalent potency as bLf 20-29 against E coli. LFB-RW and LFB-RWa had a 2-fold increase in growth-inhibitory and bactericidal activity against S aureus, compared with bLf 20-29. LFB-RI had the lowest MIC value against E coli among the peptides but lost bactericidal activity. LFB-RW and LFB-KW had stronger bactericidal activities against S aureus or E faecalis, respectively, as well as E coli than the other synthetic peptides. LFB-RF also had antibacterial activity, but this was 2-fold less than that of LFB-RW, as determined by MIC and MBC values. Conclusions and Clinical Relevance-In construction of potent antibacterial peptides, inclusion of arginine, lysine, tryptophan, or isoleucine residues enhances effectiveness against certain bacteria, as measured by MIC or MBC values.
URI: http://hdl.handle.net/11455/71598
ISSN: 0002-9645
文章連結: http://dx.doi.org/10.2460/ajvr.2003.64.1088
顯示於類別:獸醫學院

文件中的檔案:
沒有與此文件相關的檔案。


在 DSpace 系統中的文件,除了特別指名其著作權條款之外,均受到著作權保護,並且保留所有的權利。