Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/71630
標題: In vitro micropropagation of Boswellia ovalifoliolata
作者: Chandrasekhar, T.
Hussain, T.M.
Jayanand, B.
關鍵字: Boswellia
cotyledonary
regeneration
shoot regeneration
thidiazuron
期刊/報告no:: Zeitschrift Fur Naturforschung C-a Journal of Biosciences, Volume 60, Issue 5-6, Page(s) 505-507.
摘要: A protocol for micropropagation of Boswellia ovalifoliolata Bal & Henry (Burseraceae) was developed using cotyledonary nodal explant on Murashige and Skoog modified medium (MS). A comparative study of micropropagation with 6-benzyladenine, kinetin and thidiazuron along with 1-naphthalene acetic acid (0.054 mu M) was conducted. The highest shoot multiplication (7.1 +/- 0.2 shoots per node) was achieved in 50 d on MS supplemented with thidiazuron (2.72 mu M). Excised shoot cuttings of 3.0 cm were placed on the MS basal medium supplemented with indole-3-acetic acid and indole-3-butyric acid alone and in combinations for rooting. Activated charcoal (100 mg l(-1)) and polyvinylpyrrolidone (40 mg l(-1)) were added to the medium to prevent browning of cultures. The regenerated plantlets have been successfully acclimatized and transferred to soil.
URI: http://hdl.handle.net/11455/71630
ISSN: 0939-5075
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