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An automatic cultivating reactor system which can be used to continuously produce the microalgal cells has been designed and developed in this research. The system was constructed and combined together with the parts of the cultivating tank, the aeration equipment, the pH value feedback control device, the stirring device, the new cultivating solution storage unit, the solution inlet and outlet control equipment, the temperature control system, the illumination device, the outer cover unit, the PLC (programmable logical controller) automatic control system and other sensors. The valid capacities of the cultivating tank of the reactor and the storage tank of the new cultivating solution storage unit were 150 and 120 litters, respectively. Through the unloading tests with the set of continuous illumination, the actual rotation speed of the stirring shaft measured was varying between 0 and 352.5 rpm and which could be adjusted and controlled using an indirect circuit frequency controller between frequencies of 0 and 60Hz. A regression equation (y=-0.04899 + 5.87296x where y denoting the rotation speed and x denoting the frequency) between the relation of rotation speed and frequency was also established. According to the need of the cultivating requirements, the air supply amount could be chosen from 0 to 4 l/min and the illumination condition could be changed to 4, 8 or 12 light sources with the illuminance of 2600, 3600 or 5000 lux, respectively. The system temperature could be maintained between 25 and 29℃ while the reactor temperature was set at 27℃. The pH level was also held between the range of 6.5 and 7.5 as controlled by injecting an appropriate amount of the CO2 gas. The optimal cultivating capacity of the reactor was found to be 120 litters. Based on the actual pilot cultivating tests of Isochrysis aff. galbana under continuous illumination, the reactor system of microalgae developed in this research demonstrated the function of automatic semicontinuous cultivations. The pilot testing results also showed that the daily dilution ratio 25% obtained the better cultivation performance. Furthermore, the results obtained from the pilot tests of batch culture using the reacter system also showed that within three days, the density of the algal cells could be achieved up to 1193×10^4 cells/ml.
|Appears in Collections:||第58卷 第04期|
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