Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/81010
標題: 以網版印刷碳電極製作類克拉克式溶氧感測器的研發
Clark-Like+Dissolved+Oxygen+Sensors+Based+on+the+Screen+Printed+Carbon+Electrodes
作者: Lin, Shiau-An
林孝安
Wu, Ching-Chou
吳靖宙
關鍵字: Screen printed carbon electrodes
電沉積金奈米層
Clark-like dissolved oxygen sensors
Au-electrodeposited nanolayer
網版印刷碳電極
類克拉克溶氧感測器
出版社: 臺中巿: 國立中興大學農學院
摘要: In the fermentation industries, the bioreactor can achieve the best product yield by optimizing the growth conditions of microorganisms. Especially for aerobic bacteria, the change in the dissolved oxygen concentration may significantly influence the product formation of microorganisms. Therefore, it is an important issue to develop a long-term, stable, cost-effective and specific oxygen sensor. In this study, an Au nanolayer-electrodeposited screen printed carbon electrode (SPCE) acted as the working electrode was coated by the polydimethylsiloxane (PDMS) emulsion to form a Clark-like dissolved oxygen sensor. The modification of 2-[methoxy(polyethylenxy)-ropyl] trimethoxysilane (PEG-silane) can reduce the poisoning of albumin to the PDMS surface of SPCE but let the 90% response time increase from 35.6 s to 40.7 s. Moreover, the electrodes can obtain a good linearity (R^2=0.9935) in the range of 0.09-4.75 ppm. After three-day soaking in phosphate buffer solution, the oxygen permeability of PDMS membrane could present stable. After measuring for 30 days, the current discontinuously measured once one day only decreased to 88.39±2.28% of the original value. The results show that the electrodes can drastically reduce the fabricating cost and procedures compared to the presently used Clark oxygen electrode, and possess at least 30 day life time. The Clark-like electrodes have a great potential capable of being used in a bioreactor for the monitoring of oxygen consumption.
在發酵工業製程上,可藉由微生物生長條件的最適化,以達到生物反應器的最佳產率。特別對嗜氧性微生物而言,溶氧濃度的改變會顯著影響微生物代謝產物的形成,因此開發可長期、穩定、價格便宜與專一的溶氧感測器是項重要的議題。本研究以電沈積金奈米層之網版印刷碳電極(screen printed carbon electrode,SPCE)當工作電極,並塗佈聚二甲基矽氧烷(polydimethylsiloxane,PDMS)乳膠在SPCE上,以形成類克拉克溶氧電極。此電極經2-[methoxy(polyethylenxy)-propyl]trimethoxysilane(PEG-silane)修飾後,可顯著抑制白蛋白對PDMS 透氧膜的毒化,然而經PEG-silane修飾後的電極其90%響應時間從35.6 s增加至40.7 s,此電極在0.09-4.75 ppm氧濃度呈現一良好線性關係(R^2=0.9935)。在長期穩定度的測試中,發現先經三天浸泡處理的時間,可使PDMS膜的氧氣通透率趨於穩定。在一天一次的間斷式量測下,經30天後其電流值僅下降至初始值的88.39±2.28%。此電極的開發可大幅降低現有之克拉克式溶氧電極的製作成本與程序,並具有至少30天的使用壽命,此結果顯示此電極具有可應用於生物反應器內進行氧氣消耗監測的潛力。
URI: http://hdl.handle.net/11455/81010
Appears in Collections:第60卷 第03期
農業暨自然資源學院

文件中的檔案:

取得全文請前往華藝線上圖書館



Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.