Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/81151
標題: 檸檬香蜂草及甜羅勒藥用植物增殖系統之建立
Establishment of multiplication protocols on medicinal plants of Melissa officinalis L. and Ocimum basilicum L.
作者: 何信賢
Ho, Shin-Shien
關鍵字: 檸檬香蜂草
Melissa officinalis L.
甜羅勒
藥用植物
增殖系統
Ocimum basilicum L.
medicinal plants
multiplication protocols
出版社: 生命科學院碩士在職專班
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摘要: 本試驗利用微體繁殖方法,建立檸檬香蜂草及甜羅勒兩種藥用植物的增殖系統,過程包含建立無菌培植體、芽體誘導及發根。檸檬香蜂草之芽體誘導試驗係以生長調節劑BA/NAA=10/1的比例,濃度分別為BA 0.1 mg/L加NAA 0.01 mg/L、BA 0.2 mg/L加NAA 0.02 mg/L、BA 0.4 mg/L加NAA 0.04 mg/L、BA 0.6 mg/L加NAA 0.06 mg/L及BA 0.8 mg/L加NAA 0.08 mg/L的MS培養基作為試驗組;未添加任何生長調節劑的MS培養基作為對照組,結果顯示檸檬香蜂草培養於MS培養基添加BA 0.1 mg/L和NAA 0.01 mg/L,可獲得最多芽數,但添加生長調節劑BA會抑制植物生長,植株高度較矮小;檸檬香蜂草發根試驗以MS、1/2 MS、1/2 MS加IBA 0.2 mg/L、1/2 MS加IBA 0.5 mg/L、1/2 MS加NAA 0.2 mg/L、1/2 MS加NAA 0.5 mg/L進行試驗,結果顯示1/2 MS培養基為最適合的發根培養基,培養一週發根率為100.0 %。甜羅勒芽體誘導試驗以生長調節劑BA/NAA=10/1的比例,濃度分別為BA 0.1 mg/L加NAA 0.01 mg/L、BA 0.2 mg/L加NAA 0.02 mg/L、BA 0.4 mg/L加NAA 0.04 mg/L、BA 0.6 mg/L加NAA 0.06 mg/L及BA 0.8 mg/L加NAA 0.08 mg/L的MS培養基作為試驗組;未添加任何生長調節劑的MS培養基作為對照組,結果顯示甜羅勒培養於MS培養基添加BA 0.1 mg/L和NAA 0.01 mg/L,可獲得最多芽數;甜羅勒發根試驗以MS、1/2 MS、1/2 MS加IBA 0.2 mg/L、1/2 MS加IBA 0.5 mg/L、1/2 MS加NAA 0.2 mg/L、1/2 MS加NAA 0.5 mg/L進行試驗,結果顯示以1/2 MS培養基為最適合的發根培養基,第一週發根率達到100.0 %。
The aims of this study were to establish in vitro multiplication protocol of Melissa officinalis L. and Ocimum basilicum L. The processes include establishing aseptic explants,inducing shoots, rooting and transplanting. In the shoots inducing test, the medium of Melissa officinalis L. were BA 0.1 mg/L+NAA 0.01 mg/L, BA 0.2 mg/L+NAA 0.02 mg/L, BA 0.4 mg/L+NAA 0.04 mg/L,BA 0.6 mg/L+NAA 0.06 mg/L, BA 0.8 mg/L+NAA 0.08 mg/L, with BA-NAA ratio 10:1 and the Murashige and Skoog medium (MS) was the control group. In vitro micropropagation of Melissa officinalis L. was established to get maximum shoots multiplication rate by cultivating axillary buds on Murashige and Skoog medium (MS) supplemented with BA 0.1 mg/L+ NAA 0.01 mg/L, but added plant growth regulators suppressing growth of explants.In the rooting test, the medium of Melissa officinalis L. were MS, 1/2 MS, 1/2 MS with IBA 0.2 mg/L, 1/2 MS with IBA 0.5 mg/L, 1/2 MS with NAA 0.2 mg/L and 1/2 MS with NAA 0.5 mg/L. Rooting rate achieved 100.0 % by cultivating on 1/2 MS medium for 1 weeks. In the inducing shoots test, the medium of Ocimum basilicum L. were BA 0.1 mg/L+NAA 0.01 mg/L, BA 0.2 mg/L+NAA 0.02 mg/L, BA 0.4 mg/L+NAA 0.04 mg/L, BA 0.6 mg/L+NAA 0.06 mg/L, BA 0.8 mg/L+NAA 0.08 mg/L with BA-NAA ratio 10:1 and the Murashige and Skoog (MS) medium was the control group. In vitro micropropagation of Ocimum basilicum L. was established to get maximum shoots multiplication rate by cultivating axillary buds on Murashige and Skoog (MS) medium supplemented with BA 0.1 mg/L+ NAA 0.01 mg/L. In the rooting test, the medium of Ocimum basilicum L. were MS, 1/2 MS, 1/2 MS with IBA 0.2 mg/L, 1/2 MS with IBA 0.5 mg/L, 1/2 MS with NAA 0.2 mg/L and 1/2 MS with NAA 0.5 mg/L. Rooting rate achieved 100.0 % by cultivating on 1/2 MS for 1 weeks.
URI: http://hdl.handle.net/11455/81151
其他識別: U0005-3101201315331000
文章連結: http://www.airitilibrary.com/Publication/alDetailedMesh1?DocID=U0005-3101201315331000
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