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|標題:||The Use of Guava Juice in the Culture Medium of Orchid|
The objective of this study was to investigate the respective effects of guava fruit juice and coconut milk on the seed germination and growth of orchids. Seeds of Cattleya and Dendrobium phalaenopsis hybrid, seeds and unrooted seedlings of Spathoglottis plicata were used. High seed germination rate and vigorous growth of seedlings were obtained in a medium containing NAA plus BA, or coconut milk. Results also showed the effectiveness of guava fruit juice in promoting growth rate of seedlings was in the order of mesocarp＜ endocarp＜ exocarp. Results observed were:(1) Auxin-like substances were found in both fruits. In guava fruit, the highest concentration was found in endocarp, mesocarp the next, and exocarp the lowest. Auxin concentration in coconut milk was found low. (2) Growth inhibitory substances was rich in exocarp of guava fruits. (3) Coconut milk was a rich source of cytokinin, guava exocarp and mesocarp next in concentration while endocarp had the lowest. (4) Coconut milk was low in tannin content and guava fruits high, especially in the exocarp had the least. Due to the relatively stronger inhibitory effects in guava fruit juice, presumably caused by the presence of growth inhibitor and tannin, guava fruit juice was not considered as an suitable component of the MS medium. Activated charcoal added to the medium promoted the germination of seeds and enhanced the growth of orchid seedlings. This phenomenon might be due to the fact that activated charcoal absorbed the poisonous substances and tannin from the medium, thus relieved the effects of inhibitors. In conclusion, we suggest that guava fruit juice should not be used as an additive to any germination or transplanting medium for orchids unless activated charcoal is added.
|Appears in Collections:||第03期|
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