Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/81504
標題: 橫山梨及新興梨組織培養枝梢發根方法之探討
Studies on the Rooting of Hengshan and Sinkou Pear Shoots in Vitro Culture
作者: 王芳美
Fang-May Wang
郭銀港
楊耀祥
Yin-Kang Kuo
Yau-Shiang Yang
關鍵字: Hengshan Pear
橫山梨
Sinkou Pear
In Vitro Culture
Rooting
新興梨
組織培養
發根
摘要: 本試驗係探討橫山梨及新興梨組織培養枝梢之最適合發根培養方法,以改進其發根率而達到大量迅速繁殖之目的。 橫山梨以繼代培養7代以上,5片展開葉、長4-5cm之培養枝梢、扦插1mg/1IBA之1/2MS培養基中,6日後再移至不含auxin之1/2MS培養基;新興梨則以繼代培養7代以上,5片展開葉、長2.5-3.5cm之培養枝梢,扦插於添加1mg/1NAA之1/2MS培養基中,暗處理6日後移至不含auxin之1/2MS培養基,或以50mg/1IBA溶液浸漬24小時後再扦插於不含auxin之1/2MS培養基,枝梢之發根率、根數、根長最佳。
The effect of culture methods on rooting of 'Hengshan' and 'Sinkou' per Pyrus pyrifloia Nakai shoots in vitro were studied to establish a complete mass multiplication technique. It was found that rooting for 'Hengshan' pear shoot should be used 4-5 cm in length with 5 expanding leaves subcultured for 7 times and planted on the 1/2 MS medium containing 1mg/l IBA in the light condition for 6 days, then transfered to 1/2 MS medium without auxin. While, 'Sinkou' pear shoot should be used 2.5-3.5 cm in length with 5 expanding leaves, subcultured for 7 times, and planted on 1/2 MS medium with 1mg/l NAA in dark condition for 6 days, then transfered to 1/2 MS medium without auxins; the alternative way was dipped in a 50mg/l IBA solution for 24 hrs and then planted to 1/2 MS medium without auxins.
URI: http://hdl.handle.net/11455/81504
ISSN: 0255-5921
Appears in Collections:第17期
園藝學系

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