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|標題:||Evaluation of Waxapple Leaf Viability Using Improved 2,3,5-Triphenyltetrazolium Chloride Reduction Method|
Reduction of 2,3,5-triphenyltetrazolium chloride (TTC) to form insoluble red colored triphenylformazane (TPF) reaction was conventionally used to detect seed, bud, leaf and cellviability. The conventional method using 95% alcohol to extract all pigment including, chlorophyll and TPF that dissolved or suspended in alcohol solution and read at 485nm for TPF. More refinement of the viability comparison between treatment need further extraction, purification as well as quqntification of the reaction production TPF. Furthermore, the reaction production, TPF is water insoluble, but soluble in normal hexane, thus it can be partitioned into organic phase after 50% alcohol extraction, leaving the interfering pigment, chlorophyll in water phase. this treatment makes interfering factor such as green tissue which often cause browing after extraction to a minimum. Thus TPF can be read with interference free at its absorption maximum of 492nm.
|Appears in Collections:||第22卷 第02期|
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