Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/81614
標題: 金線蓮以液體與固體交互培養進行大量繁殖之研究
Studies on In vitro Propagation of Anoectochilus formosanus Hayata. by Interchange Culture with Liquid Medium and Agar Medium for Mass Propagation
作者: 許家言
Mu-Dan Chu
邱木丹
Jia-Yan Hsu
關鍵字: Anoectochilus formosanus
金線蓮
in vitro propagation
Interchange culture
Mass propagation
組織培養
液體與固體交互培養
大量繁殖
摘要: 利用組織培養技術進行金線蓮莖節段培養之大量繁殖程中,繁殖速率緩慢是最大的瓶頸問題。使用液體與固體交互培養,可在短時間內大量增殖芽數,且可使幼苗莖幹粗壯,生長良好,而無幼芽叢生細小等問題,且可避免組織玻璃質化的現象,是值得推廣的有效大量繁殖方法。
The purpose of this study was to develop an effective method for mass propagation of Anoectochilus formosanus Hayata. through tissue culture techniques. The effectiveness of various explant size and medium phase were investigated. Experimental results showed that the propagation rate was higher in liquid culture than soild agar medium. Otherwise, interchange culture with liquid medium and agar medium was the most rapid propagatative method of all because of mass propagation rate, study growth and no vitrification symptom, will be developed potentially for mass propagation of Anoectochilus formosanus Hayata in the future.
URI: http://hdl.handle.net/11455/81614
ISSN: 0255-5921
Appears in Collections:第24卷 第04期
園藝學系

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