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Effects of Flooding on the Protein Synthesis of Sponge Gourd (Luffa spp.)
2-D gel electrophresis
|摘要:||The root fragments and leaves discs of 'Seven beauty' and 'viva beauty' cultivars of sponge gourds were used to study the changes in protein synthesis under various flooding duration. Protein synthesis was mwasured by 35S-methionine incorporation and analyzed by two-dimensional sodium lauryl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and fluorography. The labeled polypeptides profiles of non-flooding control and treatments of different flooding duration (1/2, 1, 3 and 5 days) were quite similar. There were 11, 13, 20, and 15 of induced polypeptides in leaves of 'seven beauty' sponge gourds, 2,4,3, and 2 of induced polypeptides in roots, and 11, 8 ,16, and 16 of repressed polypeptides in leaves after 1/2, 1, 3 and 5 days of flooding, respectively. There were 14, 10, 13, and 12 of induced polypeptides in leaves of 'viva beauty' sponge gourd, 3, 5, 4 and 4 ofinduced polypeptides in roots, and 4, 12, 11, and 12 of repressed polypeptides in leaves, after 1/2, 1, 3, and 5 days of flooding, respectively. The changes of those polypeptides among treatment can be classified into five group. Group 1 polypeptides were enhanced or repressed only at certain flooding period. Group 2 polypeptides were remained enhancement or repression for 1 day of flooding. group 3 polypeptides were unique to flooding stress. Group 4 polypeptides were decreased but not repressed completely under flooding. Group 5 polypeptides were unique to aerobic environment.|
|Appears in Collections:||第26卷 第04期|
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