請用此 Handle URI 來引用此文件: http://hdl.handle.net/11455/81736
標題: Effect of Transplant Timing on Plantlet Quality ex vitro of Doritaenopsis
朵麗蝶蘭組培苗出瓶期對瓶苗品質生育之影響
作者: 徐淑芬
Shu-Fen Hsu
林瑞松
Ruey-Song Lin
關鍵字: 朵麗蝶蘭
Root senescence
出瓶時期
二氧化碳
根部老化
乙烯
Ethylene
Carbon dioxide
Transplanting timing
Doritaenopsis
摘要: 本試驗主要探討最佳的出瓶時期及不同苗齡瓶苗出瓶後之生長與發育。朵麓蝶蘭瓶苗,繼代後第30、60、90及120天偵測瓶內氣體的濃度。結果顯示,二氧化碳隨著培養天數增加而下降,以120天0.6%為最低值。且各生長階段皆有日夜消長變化,明期開始時二氧化碳逐漸下降;進入暗期後二氧化碳開始上升至暗期結東。而乙烯生成量隨著瓶苗株齡增加而升高,繼代後期(90、120天)瓶內乙烯的濃度皆為0.2ppm,比30及60天高達10倍,且出現盤根、根部老化,根部失去向地性、毛狀根及革質根。以TTC測定根部活力,瓶苗在定瓶後期出現根部老化、活力下降現象。 分析相對生長量,顯示朵麗蝶蘭在不同苗齡出瓶後恢復生長勢之能力有顯著差異。在鮮重及乾重方面,皆以60天苗齡瓶苗出瓶後之相對生長量最快。因此根據瓶內二氧化碳、乙烯濃度、根部活性及相對生長量之試驗結果,朵麗蝶蘭Doritaenopsis I-Hsin New Girl 'KH5250'在定瓶後60天,呈現最低老化與逆境反應。
The studies were to seek optimum timing for ex vitro transplant, effect of plantlet age on the growth of plants which were ex vitro transplanted. Doritaenopsis I-Hsin New Girl 'KH5250'determined content of gas in the flask decreased along with increase of growing days, 120 days after subculture was lowest and the value was 0.6%. There was diurnal rhythm of carbon dioxide content in the flask for every stage. The carbon dioxide content decreased at the start of light period and increased gradually during dark period. Ethylene content in cultural vessels arose with increases of plantlet age. Ethylene content was more ten-fold increase at 90 and 120 days after subculture and the values were both 0.2 ppm. At the same time, plantlets appeared root circling, root senescence, loss of the geotropism, hairy root and coriaceous root. The root senescence and activity down was observed at 90 and 120 days after subculture by the TTC determination way. There was significantly different from restoring force of growing ability at different plantlet age after ex vitro transplant for analysis of relative growth weight. After analyzing fresh weight and dry weight, found the best of growth rate were both 60 days after ex vitro transplanting. According to the carbon dioxide and ethylene content in the flask, root activity, relative growth weight, the lowest aging and adverse circumstance response at 60 days after subculture.
URI: http://hdl.handle.net/11455/81736
ISSN: 0255-5921
顯示於類別:第29卷 第01期
園藝學系

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