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Effect of Support in the Growth of Phalaenopsis Seedlings in vitro
Microporous membrane raft
In this study, non-woven fabric(L-N), filter paper(L-P)and microporous membrane raft(L-R)were used as supports in GA-7 vessels with liquid medium, containing Knudson C (1946)basal salts, 34 g L-1 potato and 20 g L-1 sucrose, for phalaenopsis seed culture. As 50 ml liquid medium was used per vessel, over 70% hyperhydric seedlings were observed after 75 days cultured in L-N or L-R. After transplanted to agar solidified medium for 90 days, hyperhydration rate of seedlings from L-N culture decreased to 3% and 70% of total seedlings developed into normal seedlings. As 40 ml liquid medium was used per vessel, hyperhydration rate was at 3% after 75 days of sowing as non-woven fabric covered with paper towel(L-NC)was used as support. The fresh and dry weight and of seedlings cultured on L-NC support were higher than that culture on solid medium. Seedlings from L-NC culture transplanted to liquid medium with L-NC support grew more leaves than that cultured on solid medium or cultured on liquid medium with vivid cube as support.
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