Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/81763
標題: 利用形態性狀及RAPD分子誌標分析Xanthasoma遺傳歧異度之研究
Genetic Diversity in Xanthosoma Based on Morphological Traits,RAPD and SSR
作者: 鄭思敏
See-Min Tee
宋妤
Yu Sung
關鍵字: genetic diversity
逢機增殖多型性
germplasm
RAPD
Xanthosoma

種源
遺傳相似度
摘要: The genetic diversity of 14 Xanthosoma accessions were evaluated by morphological traits and RAPD (randomly amplified polymorphic DNA), The morphologies among accesssions were indistinguishable except colors of leaf blades, leaf veins, petioles corm shape, colors of buds and flesh of KCX001 and 002 accessions. The KCX002 accessions was lost during plantation. The rest were clustered into 3 groups by UPGMA cluster analysis with genetic similarity 0.70~0.87. KCX001 was the only accession in 3rd group wuth similarity 0.70. The 2nd group included KCX003, 005,006,007 and 0.13 with similarity 0.87. Twenty-nine out of 88 primers that amplified reproducible polymorphic band were selected for RAPD analysis. The polymorphism was 42.3% with an average of 2.8 polymorphic bands per primer. The cluster analysis result 3 groups with KCX002 comprised the first with genetic similarity 0.64. KCX001 was the only accession in 3rd group. The other accessions had high genetic similarity values of 0.99 and comprised3d the 2nd group. The result reflected that many of Xanthosoma accessions in this study were actually duplicates.
本實驗利用形態性狀及RAPD分子標誌鑑定14個Xanthosoma收集系之遺傳歧異度分析,形態性狀調查結果,除KCXOOl及002葉面、葉脈中心點顏色、葉脈及葉柄,KCXOOl之母芋形狀、芽眼色及肉色性狀外,其他13個收集系之外觀無法以肉眼進行區分。進行群叢分析結果,KCXOO2因生育後期種原遺失,其餘13個收集系之遺傳相似度為0.70~0.87之3群組,以CX001獨立成0.70之第三群組,KCX003、005、006、007及013以0.79之相似度組成第二群組,餘者組成0.87相似度之第一群組。自88個逢機引子選出29個具多型性之引子,平均每個引子可產生6.5個增幅條帶,具多型性者有2.8個,多型性為42.3%。群叢分析結果共分成3群組,第一群組由KCXOO2以0.64之遺傳相似度獨立組成,KCXOOl則以O.86之遺傳相似度組成第三群,其餘收集系以0.99以上之相似度組成第二群組。本研究結果顯示受測之Xanthosoma收集系於基因型上有高度重複的情形,
URI: http://hdl.handle.net/11455/81763
ISSN: 0255-5921
Appears in Collections:第30卷 第01期
園藝學系

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