Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/84816
標題: Fermentation approach for enhancing 1-butanol productionusing engineered butanologenic Escherichia coli
關鍵字: E. coli
1-Butanol
OGAB
Fermentation approach
摘要: In this study, engineered butanologenic Escherichia coli T5 constructed by the OGAB method was used for1-butanol production. The results showed the feasibility of the artificial butanologenic operon, (PromoterPr)-thil-crt-bcd-etfB-etfA-hbd-adhe1-adhe, where the 1-butanol titer, specific BuOH yield, and BuOH yieldwere 4.50 mg/L, 4.50 mg-BuOH/g cell, and 0.35 mg-BuOH/g-glucose, respectively. Fermentation conditionsof anaerobic, low initial concentrations of carbon sources, low oxidation state of carbon source,pH of 6, addition of glutathione and citrate, had been shown for efficiently improving the 1-butanol production.The premise behind these fermentation approaches can be categorized into two lines of reasoning,either elevated the availability of acetyl-CoA or lowered the intracellular redox state. By comparingthe fermentation conditions tested in this study, pH has been shown to be the most efficiency strategiesfor 1-butanol production while the replacement of glucose with glycerol provides the highest improvementin butanol yield.
URI: http://hdl.handle.net/11455/84816
文章連結: http://dx.doi.org/10.1016/j.biortech.2013.01.115
Appears in Collections:化學工程學系所

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