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dc.creatorJian-Ren Chenen_US
dc.creatorJia-Jiuan Wuen_US
dc.creatorJustin Chun-Te Linen_US
dc.creatorYuan-Chuen Wangen_US
dc.creatorChiu-Chung Youngen_US
dc.creatorChwen-Jen Shiehen_US
dc.creatorShih-Lan Hsuen_US
dc.creatorCheih-Ming J. Changen_US
dc.description.abstractIn this study, supercritical anti-solvent (SAS) pulverization coupled with reverse phase elution chromatography was employed to isolate 9-cis and trans-β-carotenes from Dunaliella salina. Total concentration of 9-cis (134.7 mg/g) and trans-β-carotene (204.2 mg/g) was increased from 338.9 mg/g of the ultrasonic extract to 859.7 mg/g (338.9 for 9-cis and 520.8 for trans) of the elution fraction. The SAS pulverization of the collected fraction further produced submicron-sized particulates containing 932.1 mg/g (355.6 for 9-cis and 576.5 for trans) of total β-carotenes with a recovery of 86.3% (83.9% for cis and 87.8% for trans). Effects of two SAS operational conditions on the purity, recovery of total β-carotenes, mean size and morphology of the precipitates were obtained from an experimentally designed method. Generation of micronized particulates enriched with 9-cis and trans-β-carotenes by low-density SAS was proved to be feasible and environmental benign.en_US
dc.relationJournal of Chromatography A, Volume 1299, Page(s) 1-9.en_US
dc.subject9-cis-β-Carotene, trans-β-caroteneen_US
dc.subjectSupercritical anti-solvent Pulverizationen_US
dc.subjectElution chromatographyen_US
dc.titleLow density supercritical fluids precipitation of 9-cis and all trans-β-carotenes enriched particulates from Dunaliella salinaen_US
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