Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/84970
標題: Direct visualization of the quadruplex structures in human chromosome using FRET: Application of quadruplex stabilizer and duplex-binding fluorophore
關鍵字: G-quadruplexes
Telomere
Chromosome
Fluorescent mapping
Fluorescence resonance energy transfer (FRET)
摘要: The G-quadruplex structures in the telomere of a chromosome can not only protect the internal chromosome sequences by preventing the improper activation of DNA-damage-response pathways but also become targets for cancer treatments. In this manuscript, we wish to prove the existence of G-quadruplex structure formation, rather than G-quadruplex sequence, in chromosome of human cancer cells. Based on our studies, the fluorescent mapping of G-quadruplex structures in the chromosome is possible with the combination of G-quadruplex targeting fluorophore (BMVC, 3, 6-bis-(1-methyl-4-vinylpyridinium)-carbazole diiodide) and duplex-binding fluorophores (Hoechst or propidium iodide). By means of an applicable incubation time between cell cycle period and proper staining procedure to the chromosome, FRET (fluorescence resonance energy transfer) between G-quadruplex targeting fluorophore and duplex-binding fluorophore can increase the signal contrast of the fluorescent color and the fluorescent mapping of quadruplex structures can be easily observed using fluorescence microscopy. These observations are further supported by basic spectral analysis, titration binding assay, gel electrophoresis binding competition assay and confocal microscopy.
URI: http://hdl.handle.net/11455/84970
文章連結: http://dx.doi.org/10.1016/j.bios.2013.03.041
Appears in Collections:生醫工程研究所

文件中的檔案:

取得全文請前往華藝線上圖書館



Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.