Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/85182
標題: Invadopodia-associated proteins blockade as a novelmechanism for 6-shogaol and pterostilbene to reducebreast cancer cell motility and invasion
關鍵字: Breast cancer
Invadopodia
Metastasis
Pterostilbene
6-Shogaol
摘要: AbstractSCOPE:Invadopodia are actin-rich membrane protrusions of tumor cells that are thought to initiate the local migration and invasion during cancer metastasis. The blockade of invadopodia-associated proteins has been reported as a promising approach for prevention of tumor metastasis. The aim of this study was to investigate the modulatory effects of 6-shogaol and pterostilbene on invadopodia in aggressive breast cancer cells.METHODS AND RESULTS:By wound-healing, transwell, and gelatin zymography assays, we found that 6-shogaol and pterostilbene effectively attenuated the motility and invasion of MDA-MB-231 cells, and suppressed the activities of matrix metalloproteinase-2 (MMP-2) and matrix metalloproteinase-9 (MMP-9). Further investigation into the underlying molecular mechanisms revealed that the levels of key modulators of invadopodium maturation, including c-Src kinase, cortactin, and membrane type 1-matrix metalloproteinase (MT1-MMP) decreased when cells were treated with 6-shogaol or pterostilbene.CONCLUSION:These data suggest that the repression of these factors might affect the maturation of invadopodia, inhibiting the metastasis of MDA-MB-231 cells. In conclusion, the present study demonstrates for the first time that 6-shogaol and pterostilbene can inhibit invadopodium formation and MMP activity in highly invasive breast cancer cells. We suggest that these compounds may be clinically useful in chemopreventive treatments for metastatic breast cancer.
URI: http://hdl.handle.net/11455/85182
文章連結: http://onlinelibrary.wiley.com/doi/10.1002/mnfr.201200715/abstract
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