Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/85207
標題: A molecular diagnosis method using real-time PCRfor quantification and detection of Fusarium oxysporumf. sp. cubense race 4
關鍵字: Fusarium oxysporum f
sp
cubense
Fusarium wilt
Molecular detection
Reliablediagnosis
Real-time PCR
SCAR
摘要: The Fusarium genus causes devastatingplant diseases worldwide, in which Fusarium oxysporumis the most serious crop pathogen. Disease monitoringis the basis of integrated pest management ofany disease. The lack of rapid, accurate, and reliabledevice to detect and identify plant pathogens is one ofthe main limitations in integrated disease management.This study describes an efficient and quantifiable diagnosismethod for the specific detection of F. oxysporumf. sp. cubense (Foc) race 4 in field-infected banana.With the optimized PCR parameters using the SCAR(sequence characterized amplified region) primersFocSc-1/FocSc-2 and a real-time PCR strategy, thedeveloped method showed high reproducibility andwas very sensitive to detect extremely low quantitiesof Foc genomic DNA (gDNA).We also found that FocgDNA in severely symptomatic banana pseudostemsand leaves were 6946-fold and 26.69-fold more than inthose of mild-symptomatic banana, respectively.
URI: http://hdl.handle.net/11455/85207
文章連結: http://link.springer.com/article/10.1007%2Fs10658-012-0096-0
Appears in Collections:植物病理學系

文件中的檔案:

取得全文請前往華藝線上圖書館



Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.