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標題: Supercritical Carbon Dioxide Micronization of Zeaxanthin fromModerately Thermophilic Bacteria Muricauda lutaonensis CC-HSB-11T
關鍵字: Muricauda lutaonensis
supercritical carbon dioxide
dynamic light scattering
摘要: Moderately thermophilic bacterial strain CC-HSB-11T (Muricauda lutaonensis), which was described recently from acoastal hot spring of Green Island, Taiwan, has been identified to produce zeaxanthin as a predominant xanthophyll by liquidchromatography tandem mass spectrometry (LC MS/MS). Cell culture in bioreactor produced 3.12 ( 0.18 mg zeaxanthin L 1of culture. Micronization of zeaxanthin was achieved through supercritical carbon dioxide antisolvent precipitation method. Yield ofzeaxanthin after the process was 53.4%. Dynamic light scattering assay determined the polydisperse existence of micronized particlesof size 3 nmto 2 μm. Field emission scanning electron microscopy revealed distinct morphology and size distribution heterogeneityof particles. Integrity of zeaxanthin after the antisolvent process was assessed by LC MS/MS. The technique capitalizes on theinherent ability of CC-HSB-11T to synthesize zeaxanthin and the work demonstrated feasibility of antisolvent precipitation methodto produce microparticles exploiting a bacterial strain.
Appears in Collections:土壤環境科學系



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