請用此 Handle URI 來引用此文件: http://hdl.handle.net/11455/85257
標題: Description of Noviherbaspirillum malthae gen. nov.,sp. nov., isolated from an oil-contaminated soil, andproposal to reclassify Herbaspirillum soli,Herbaspirillum aurantiacum, Herbaspirillumcanariense and Herbaspirillum psychrotolerans asNoviherbaspirillum soli comb. nov., Noviherbaspirillumaurantiacum comb. nov., Noviherbaspirillumcanariense comb. nov. and Noviherbaspirillumpsychrotolerans comb. nov.based on polyphasic analysis
摘要: An aerobic, Gram-negative, rod-shaped bacterium with polar flagella, strain CC-AFH3T, was isolated from an oil-contaminated site located in Kaohsiung county, Taiwan. Strain CC-AFH3T grew at 20–40 °C, pH 5.0–10.0 and <2 % (w/v) NaCl. 16S rRNA gene sequence analysis indicated that strain CC-AFH3T showed the greatest degree of similarity to Herbaspirillum soli SUEMI10T (96.5 %), H. aurantiacum SUEMI08T (96.3 %), H. canariense SUEMI03T (96.0 %), H. psychrotolerans PB1T (95.4 %) and members of other Herbaspirillum species (94.1–95.2 %), and lower similarity to members of other genera (<94 %). Phylogenetic analyses also positioned the novel strain in the genus Herbaspirillum as an independent lineage. The major fatty acids in strain CC-AFH3T were C10 : 0 3-OH, C12 : 0, C14 : 0 2-OH, C16 : 0, iso-C15 : 0 3-OH, C17 : 0 cyclo, C16 : 1ω7c/C16 : 1ω6c and C18 : 1ω7c/C18 : 1ω6c. The major polar lipids of strain CC-AFH3T were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol and phosphatidylcholine. The predominant quinone was ubiquinone 8 (Q-8) and the DNA G+C content was 63.4 mol%. On the basis of 16S rRNA gene sequence analysis in combination with physiological and chemotaxonomic data, strain CC-AFH3T represents a novel species in a new genus, for which we propose the name Noviherbaspirillum malthae gen. nov., sp. nov.; the type strain of Noviherbaspirillum malthae is CC-AFH3T ( = BCRC 80516T = JCM 18414T). We also propose the reclassification of Herbaspirillum soli, Herbaspirillum aurantiacum, Herbaspirillum canariense and ‘Herbaspirillum psychrotolerans’ as Noviherbaspirillum soli comb. nov. (type strain SUEMI10T = LMG 26149T = CECT 7840T), Noviherbaspirillum aurantiacum comb. nov. (type strain SUEMI08T = LMG 26150T = CECT 7839T), Noviherbaspirillum canariense comb. nov. (type strain SUEMI03T = LMG 26151T = CECT 7838T) and Noviherbaspirillum psychrotolerans comb. nov. (type strain PB1T = DSM 26001T = LMG 27282T), respectively. An emended description of Herbaspirillum seropedicae is also presented.
URI: http://hdl.handle.net/11455/85257
文章連結: http://ijs.sgmjournals.org/content/63/Pt_11/4100.long
顯示於類別:土壤環境科學系

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