Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/86425
標題: Antihelminthic niclosamide modulates dendritic cells activation and function
作者: Wu, Chieh-Shan
Li, Yi-Rong
Chen, Jeremy J W
Chen, Ying-Che
Chu, Chiang-Liang
Pan, I-Hong
Wu, Yu-Shan
Lin, Chi-Chen
關鍵字: Contact hypersensitivity (CHS)
Cytokine
Dendritic cells
Niclosamide
T cell proliferation
Animals
Anthelmintics
Bone Marrow Cells
Cell Proliferation
Cells, Cultured
Coculture Techniques
Dendritic Cells
Dinitrofluorobenzene
Female
Gene Expression Regulation
Hypersensitivity
Immunization
Immunomodulation
Injections, Intravenous
Lipopolysaccharides
Lymphocyte Activation
MAP Kinase Kinase 4
Mice
Mice, Inbred C57BL
Mitogen-Activated Protein Kinase Kinases
NF-kappa B
Niclosamide
Signal Transduction
T-Lymphocytes
摘要: Dendritic cells (DCs) link the sensing of the environment by the innate immune system to the initiation of adaptive immune responses. Accordingly, DCs are considered to be a major target in the development of immunomodulating compounds. In this study, the effect of niclosamide, a Food and Drug Administration-approved antihelminthic drug, on the activation of lipopolysaccharide (LPS)-stimulated murine bone marrow-derived DCs was examined. Our experimental results show that niclosamide reduced the pro-inflammatory cytokine and chemokine expression of LPS-activated DCs. In addition, niclosamide also affected the expression of MHC and costimulatory molecules and influenced the ability of the cells to take up antigens. Therefore, in mixed cell cultures composed of syngeneic OVA-specific T cells and DCs, niclosamide-treated DCs showed a decreased ability to stimulate T cell proliferation and IFN-γ production. Furthermore, intravenous injection of niclosamide also attenuated contact hypersensitivity (CHS) in mice during sensitization with 2,4-dinitro-1-fluorobenzene. Blocking the LPS-induced activation of MAPK-ERK, JNK and NF-κB may contribute to the inhibitory effect of niclosamide on DC activation. Collectively, our findings suggest that niclosamide can manipulate the function of DCs. These results provide new insight into the immunopharmacological role of niclosamide and suggest that it may be useful for the treatment of chronic inflammatory disorders or DC-mediated autoimmune diseases.
URI: http://hdl.handle.net/11455/86425
ISSN: 1090-2163
Appears in Collections:生物醫學研究所

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