Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/86435
標題: Differential cytotoxic effects of gold nanoparticles in different mammalian cell lines
作者: Chueh, Pin Ju
Liang, Ruei-Yue
Lee, Yi-Hui
Zeng, Zih-Ming
Chuang, Show-Mei
關鍵字: AuNPs
Cell-impedance measurement
Cytotoxicity
Gold nanoparticles
In vitro assays
Animals
Apoptosis
Autophagy
Cell Proliferation
Cercopithecus aethiops
Colony-Forming Units Assay
Gold
Humans
Metal Nanoparticles
Mice
NIH 3T3 Cells
Oligonucleotide Array Sequence Analysis
Swine
Trypan Blue
Vero Cells
Toxicity Tests
摘要: Gold nanoparticles (AuNPs) possess unique properties that have been exploited in several medical applications. However, a more comprehensive understanding of the environmental safety of AuNPs is imperative for use of these nanomaterials. Here, we describe the impacts of AuNPs in various mammalian cell models using an automatic and dye-free method for continuous monitoring of cell growth based on the measurement of cell impedance. Several well-established cytotoxicity assays were also used for comparison. AuNPs induced a concentration-dependent decrease in cell growth. This inhibitory effect was associated with apoptosis induction in Vero cells but not in MRC-5 or NIH3T3 cells. Interestingly, cDNA microarray analyses in MRC-5 cells supported the involvement of DNA damage and repair responses, cell-cycle regulation, and oxidative stress in AuNP-induced cytotoxicity and genotoxicity. Moreover, autophagy appeared to play a role in AuNPs-induced attenuation of cell growth in NIH3T3 cells. In this study, we present a comprehensive overview of AuNP-induced cytotoxicity in a variety of mammalian cell lines, comparing several cytotoxicity assays. Collectively, these assays offer convincing evidence of the cytotoxicity of AuNPs and support the value of a systematic approach for analyzing the toxicology of nanoparticles.
URI: http://hdl.handle.net/11455/86435
ISSN: 03043894
1873-3336
Appears in Collections:生物醫學研究所

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