Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/86458
標題: Recombinant Derp5 allergen with αS1-casein signal peptide secreted in murine milk protects against dust mite allergen–induced airway inflammation
作者: Liu, Hsu-Chung
Pai, Shun-Yuan
Chen, Hsiao-Ling
Lai, Cheng-Wei
Tsai, Tung-Chou
Cheng, Winston T.K.
Yang, Shang-Hsun
Chen, Chuan-Mu
關鍵字: Dermatophagoides pteronyssinus
Derp5 allergen
allergic airway inflammation
signal peptide
transgenic mice
Animals
Antigens, Dermatophagoides
Arthropod Proteins
Blotting, Western
Caseins
Dermatophagoides pteronyssinus
Disease Models, Animal
Female
Inflammation
Lactalbumin
Mice
Mice, Transgenic
Milk
Phylogeny
Promoter Regions, Genetic
Protein Sorting Signals
Recombinant Proteins
Respiratory System
摘要: Recent advances in recombinant technology make transgenic animals that produce pharmaceutical proteins in their milk more feasible. The group 5 allergen isolated from Dermatophagoides pteronyssinus (Derp5) is one of the most important dust mite allergens in humans. The aims of this study were to develop transgenic mice that could secrete recombinant Derp5-containing milk and to demonstrate that ingesting recombinant milk protects against allergic airway inflammation. Two transgenes were constructed separately. The α-LA-Derp5f transgene consisted of the bovine α-lactalbumin (α-LA) promoter and full-length Derp5 cDNA. The α-LA-CN-Derp5t transgene included the α-LA promoter, a leader sequence of αS1-casein (CN), and signal peptide-truncated Derp5 cDNA. Both species of transgenic mice were confirmed to have successful transgene integration and stable germline transmission. Western blot analysis of the milk obtained from the offspring of transgenic mice demonstrated that recombinant Derp5 was secreted successfully in the milk of αLA-CN-Derp5t transgenic mice but not in that of αLA-Derp5f transgenic mice. This study provides new evidence that transgenic mice can secrete recombinant Derp5 efficiently in milk by adding a signal peptide of αS1-casein. The antigenic activity of recombinant Derp5 milk was demonstrated to have a protective effect against allergic airway inflammation in a murine model in which the ingestion of recombinant Derp5-containing milk was used as pretreatment.
URI: http://hdl.handle.net/11455/86458
ISSN: 00220302
1525-3198
Appears in Collections:生命科學系所

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