Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/86463
標題: EpCAM Induction Functionally Links to the Wnt-Enhanced Cell Proliferation in Human Keratinocytes
作者: Shen, Ching-I
Lee, Hsiu-Chin
Kao, Ying-Hsien
Wu, Chieh-Shan
Chen, Po-Hung
Lin, Shinn-Zong
Lai, Ping-Shan
Su, Hong-Lin
關鍵字: Antigens, Neoplasm
Cell Adhesion Molecules
Cell Cycle
Cell Line, Tumor
Cell Movement
Cell Proliferation
Cells, Cultured
Female
HEK293 Cells
Humans
Keratinocytes
MCF-7 Cells
Recombinant Proteins
Signal Transduction
Wnt Proteins
Wnt3A Protein
摘要: Accelerating proliferation of primary keratinocytes benefits skin autografts for severely burned patients. Wnt signal, a conserved pathway controlling cell cycle and morphogenesis in embryo, also involves in cell proliferation and tumorigenesis in adult tissues. Here the effects of Wnt signal on the growth of human interfollicular keratinocytes were investigated. We demonstrated that recombinant Wnt3a significantly promoted the growth of primary keratinocytes at a low cell density. A well-characterized GSK-3β inhibitor, BIO, activated the Wnt signals and also enhanced the colony formation of keratinocytes dose dependently. Gene expression profile of the BIO-treated keratinocytes revealed the linkage of BIO with cell mitosis and indicated that epithelial cell adhesion molecule (EpCAM), a Wnt target gene, was significantly upregulated. Compared to the sorted EpCAM⁻ keratinocytes, the EpCAM⁺ cells showed a higher proliferation rate and efficacy of colony formation. Inhibiting the EpCAM expression by shRNA attenuated the proliferation effect of BIO and the growth advantage of the EpCAM⁺ keratinocytes. These evidences emphasize the positive roles of canonical Wnt and EpCAM on the regulation of cell growth and self-renewal of human keratinocytes.
URI: http://hdl.handle.net/11455/86463
ISSN: 09636897
15553892
1555-3892
Appears in Collections:生命科學系所

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