Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/86483
標題: Overexpression of the HspL PromotesAgrobacterium tumefaciensVirulence inArabidopsisUnder Heat Shock Conditions
作者: Hwang, Hau-Hsuan
Liu, Yin-Tzu
Huang, Si-Chi
Tung, Chin-Yi
Huang, Fan-Chen
Tsai, Yun-Long
Cheng, Tun-Fang
Lai, Erh-Min
摘要: Agrobacterium tumefaciens transfers a specific DNA fragment from the resident tumor-inducing (Ti) plasmid and effector virulence (Vir) proteins to plant cells during infection. A. tumefaciens VirB1-11 and VirD4 proteins assemble as the type IV secretion system (T4SS), which mediates transfer of the T-DNA and effector Vir protein into plant cells, thus resulting in crown gall disease in plants. Previous studies revealed that an α-crystallin-type, small heat-shock protein (HspL) is a more effective VirB8 chaperone than three other small heat-shock proteins (HspC, HspAT1, and HspAT2). Additionally, HspL contributes to efficient T4SS-mediated DNA transfer and tumorigenesis under room-temperature growth. In this study, we aimed to characterize the impact of HspL on Agrobacterium-mediated transformation efficiency under heat-shock treatment. During heat shock, transient transformation efficiency and VirB8 protein accumulation were lower in the hspL deletion mutant than in the wild type. Overexpression of HspL in A. tumefaciens enhanced the transient transformation efficiency in root explants of both susceptible and recalcitrant Arabidopsis ecotypes. In addition, the reduced transient transformation efficiency during heat stress was recovered by overexpression of HspL in A. tumefaciens. HspL may help maintain VirB8 homeostasis and elevate Agrobacterium-mediated transformation efficiency under both heat-shock and nonheat-shock growth.
URI: http://hdl.handle.net/11455/86483
ISSN: 0031-949X
Appears in Collections:生命科學系所

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