請用此 Handle URI 來引用此文件: http://hdl.handle.net/11455/86509
標題: Eicosapentaenoic acid induces neovasculogenesis in human endothelial progenitor cells by modulating c-kit protein and PI3-K/Akt/eNOS signaling pathways
作者: Chiu, Shao-Chih
Chiang, En-Pei Isabel
Tsai, Shu-Yao
Wang, Fu-Yu
Pai, Man-Hui
Syu, Jia-Ning
Cheng, Ching-Chang
Rodriguez, Raymond L.
Tang, Feng-Yao
關鍵字: Akt
Eicosapentaenoic acid
Human endothelial progenitor cells
Neovasculogenesis
c-kit
eNOS
microRNA 221
Animals
Cells, Cultured
Eicosapentaenoic Acid
Endothelial Progenitor Cells
Humans
Mice
Mice, Nude
Neovascularization, Physiologic
Nitric Oxide Synthase Type III
Phosphatidylinositol 3-Kinases
Phosphorylation
Proto-Oncogene Proteins c-akt
Proto-Oncogene Proteins c-kit
Real-Time Polymerase Chain Reaction
Signal Transduction
摘要: Human endothelial progenitor cells (hEPCs) derived from bone marrow play a crucial in the prevention of ischemic injuries in the course of postnatal neovasculogenesis. Frequent fish oil (FO) consumption is reportedly associated with a significantly lower incidence of cardiovascular disease. However, the molecular mechanisms of eicosapentaenoic acid (EPA)/docosahexaenoic acid (DHA) are not well elucidated, and the beneficial effect of FO consumption on neovasculogenesis has not been demonstrated yet. In the current study, we investigated the effects of EPA/DHA and FO consumption on neovasculogenesis by using vascular tube formation assay, Western blotting, real-time polymerase chain reaction, immunohistochemical staining and Doppler imaging in both in vitro and in vivo models. The results demonstrate that EPA and DHA dose-dependently enhance the neovasculogenesis and cell migration of hEPCs in vitro. The mechanisms of action included up-regulation of the c-kit protein as well as the phosphorylation of the ERK1/2, Akt and endothelial nitric oxide synthase signaling molecules in hEPCs. Furthermore, EPA significantly suppressed the expression of microRNA 221 in vitro. In experimental animal models, FO consumption significantly induced the formation of new blood vessels (neovasculogenesis) and prevented ischemia. Taken together, it is suggested that FO consumption enhances neovasculogenesis mainly through the effects of EPA in hEPCs, thereby exerting a preventive effect against ischemic injury.
URI: http://hdl.handle.net/11455/86509
ISSN: 09552863
1873-4847
顯示於類別:食品暨應用生物科技學系

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