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標題: A dual gene-silencing vector system for monocot and dicot plants
作者: Liou, Ming-Ru
Huang, Ying-Wen
Hu, Chung-Chi
Lin, Na-Sheng
Hsu, Yau-Heiu
關鍵字: Bamboo mosaic virus
RNA-dependant RNA polymerase 6
VIGS vector
satellite RNA
satellite RNA-based vector
virus-induced gene silencing
Gene Silencing
Genetic Vectors
Green Fluorescent Proteins
Plant Leaves
Plant Proteins
Plants, Genetically Modified
RNA Replicase
RNA, Satellite
RNA, Viral
Species Specificity
摘要: Plant virus-based gene-silencing vectors have been extensively and successfully used to elucidate functional genomics in plants. However, only limited virus-induced gene-silencing (VIGS) vectors can be used in both monocot and dicot plants. Here, we established a dual gene-silencing vector system based on Bamboo mosaic virus (BaMV) and its satellite RNA (satBaMV). Both BaMV and satBaMV vectors could effectively silence endogenous genes in Nicotiana benthamiana and Brachypodium distachyon. The satBaMV vector could also silence the green fluorescent protein (GFP) transgene in GFP transgenic N. benthamiana. GFP transgenic plants co-agro-inoculated with BaMV and satBaMV vectors carrying sulphur and GFP genes, respectively, could simultaneously silence both genes. Moreover, the silenced plants could still survive with the silencing of genes essential for plant development such as heat-shock protein 90 (Hsp90) and Hsp70. In addition, the satBaMV- but not BaMV-based vector could enhance gene-silencing efficiency in newly emerging leaves of N. benthamiana deficient in RNA-dependant RNA polymerase 6. The dual gene-silencing vector system of BaMV and satBaMV provides a novel tool for comparative functional studies in monocot and dicot plants.
ISSN: 14677644
Appears in Collections:生物科技學研究所



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