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dc.contributor.authorKere, M.zh_TW
dc.contributor.authorSiriboon, C.zh_TW
dc.contributor.authorLiao, J.W.zh_TW
dc.contributor.authorLo, N.W.zh_TW
dc.contributor.authorChiang, H.I.zh_TW
dc.contributor.authorFan, Y.K.zh_TW
dc.contributor.authorKastelic, J.P.zh_TW
dc.contributor.authorJu, J.C.zh_TW
dc.description.abstractVascular endothelial growth factor is a multipotent angiogenic factor implicated in cell survival and proliferation. The objective was to determine effects of exogenous recombinant human VEGFA (or VEGFA165) in culture media on porcine oocyte maturation and parthenote development. Adding 5 ng/mL VEGFA to the culture medium improved the maturation rate of denuded oocytes (P < 0.05), although 5, 50, or 500 ng/mL did not significantly affect nuclear maturation of oocytes. Parthenotes from oocytes cultured either in in vitro maturation or in vitro culture medium supplemented with 5 or 50 ng/mL VEGFA had an improved blastocyst rate and increased total numbers of cells (P < 0.05). Moreover, those treated with 5 ng/mL of VEGFA had a higher hatched blastocyst rate (average of 121 cells per blastocyst). All VEGFA-treated oocytes had reduced apoptotic indices (P < 0.05), except for those with a higher dose (500 ng/mL) of VEGFA which had more apoptotic cells (P < 0.05). Adding 5 ng/mL VEGFA to oocytes during the last 22 h of in vitro maturation improved (P < 0.05) blastocyst rates and total numbers of cells, with reduced apoptosis indices similar to that of long-term (44 h) culture. Furthermore, Axitinib (VEGFR inhibitor) reversed the effects of VEGFA on parthenote development (P < 0.05). Follicular fluids from medium (2-6 mm) to large (>6 mm) follicles contained 5.3 and 7.0 ng/mL vascular endothelial growth factor protein, respectively, higher (P < 0.05) than concentrations in small (<2 mm) follicles (0.4 ng/mL). Also, VEGFA and its receptor (VEGFR-2) were detected (immunohistochemistry) in growing follicles and developing blastocysts. In addition, VEGFA inhibited caspase-3 activation in matured oocytes (P < 0.05). In conclusion, this is apparently the first report that VEGFA has proliferative and cytoprotective roles in maturing porcine oocytes and parthenotes. Furthermore, an optimal VEGFA concentration promoted porcine oocyte maturation and subsequent development.zh_TW
dc.relationDomestic Animal Endocrinology, Volume 49, Page(s) 60-69.zh_TW
dc.subjectPorcine oocytezh_TW
dc.subjectIn Vitro Oocyte Maturation Techniqueszh_TW
dc.subjectProtein Kinase Inhibitorszh_TW
dc.subjectVascular Endothelial Growth Factor Azh_TW
dc.titleVascular endothelial growth factor A improves quality of matured porcine oocytes and developing parthenoteszh_TW
dc.typeJournal Articlezh_TW
Appears in Collections:動物科學系


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