Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/86565
標題: Development and Bias Assessment of a Method for Targeted Metagenomic Sequencing of Marine Cyanobacteria
作者: Batmalle, C. S.
Chiang, H.-I.
Zhang, K.
Lomas, M. W.
Martiny, A. C.
關鍵字: DNA Transposable Elements
Gene Library
Metagenomics
Prochlorococcus
Seawater
Sequence Analysis, DNA
Synechococcus
摘要: Prochlorococcus and Synechococcus are the most abundant photosynthetic organisms in oligotrophic waters and responsible for a significant percentage of the earth's primary production. Here we developed a method for metagenomic sequencing of sorted Prochlorococcus and Synechococcus populations using a transposon-based library preparation technique. First, we observed that the cell lysis technique and associated amount of input DNA had an important role in determining the DNA library quality. Second, we found that our transposon-based method provided a more even coverage distribution and matched more sequences of a reference genome than multiple displacement amplification, a commonly used method for metagenomic sequencing. We then demonstrated the method on Prochlorococcus and Synechococcus field populations from the Sargasso Sea and California Current isolated by flow cytometric sorting and found clear environmentally related differences in ecotype distributions and gene abundances. In addition, we saw a significant correspondence between metagenomic libraries sequenced with our technique and regular sequencing of bulk DNA. Our results show that this targeted method is a viable replacement for regular metagenomic approaches and will be useful for identifying the biogeography and genome content of specific marine cyanobacterial populations.
URI: http://hdl.handle.net/11455/86565
ISSN: 0099-2240
1098-5336
Appears in Collections:動物科學系

文件中的檔案:

取得全文請前往華藝線上圖書館



Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.