Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/89169
標題: 劍葉文心蘭組織培養與後裔選種
Tissue culture and progeny selection of tolumnia orchid
作者: Nittaya Chookoh
夏秋美
關鍵字: 劍葉文心蘭
花梗培養
花芽培養
種間雜交
屬間雜交
tolumnias
flower stalks culture
flower bud culture
hybridization
offspring
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摘要: Tolumnia are indigenous to the Caribbean islands, and have extensive habitats in that region. Plants are miniature oncidium with triangular succulent leaves that form small fan shaped growths and lack of a pseudobulb. The hybrids present a surprising array of colors and have been hybridized for a relative short term to reach flowering size which enabled breeders to breed important advances in a few years. The aims of this experiment were developed an efficient tissue culture system for clonal propagation of tolumnias orchid and tested the effects of the plant growth regulators 6-benzyladenine (BA) and flower stalk node positions on mass propagation of Tolumnia Snow Fairy and Tolumnia GS248. After 16 weeks of culture, the 4th node position of flower stalks in Tolumnia Snow Fairy showed the highest (57.1%) shoot formation rate, which the 3rd node position is differentiation in Tolumnia GS248. While, both of them showed higher response on ½ MS salt basal medium supplemented with 4 mg/L BA. Not only flower stalk nodes but also the effects of flower stalks stage on the efficiency of PLBs induction from Tolumnia Snow Fairy's flower buds explants were examined. After 8 weeks of culture, the highest response rate of PLBs showed of 70% of the lateral flower buds in second stage when they were cultured on ½ MS salt basal medium with 4 mg/L BA. Next, plantlets establishment and transplantations derived from flower stalk node were cultured and transplanted to greenhouse condition presented 100% and 54.2% of the plantlets of Tolumnia Snow Fairy and Tolumnia GS248 survived after 90 days of transfer. Furthermore, only 3 progenies out of 4 crosses numbers were chosen that including, 3 plants of RS cross, 2 plants of 101-186 and 1 plant of 101-233 cross. This opens up the route for in vitro culture methods that does not damage the mother plants and also proved useful for future studies elucidating for floral tissues involved in subsequent differentiation processes of shoot formation and PLBs induction. Finally, the progenies were selected and hope to use in flower stems or flower buds culture for multiplying of progenies.
劍葉文心蘭原產於加勒比海群島並廣泛的分佈於該區域,帶有三角形之肉質葉,葉片成扇形排列,無假球莖之小型文心蘭,劍葉文心蘭的育種歷史相對較短,近幾年是其育種重要發展的關鍵。本論文為建立對劍葉文心蘭有效的組織培養方法,以Tolumnia Snow Fairy 及Tolumnia GS248花梗節位及不同濃度的BA進行試驗,培養16週後,Tolumnia Snow Fairy的最佳繁殖節位為第四節,而Tolumnia GS248的第三節為最佳繁殖節位,最佳培養基則為1/2 MS + 4 mg/l BA,同時也對Tolumnia Snow Fairy的不同花莖發育時期花芽對類原球體誘導之影響進行研究,以1/2 MS + 4 mg/l BA 的培養基培養第二階段的側花芽有最高的類原球體形成率( 7 0 %) 。將由花梗芽長成的植株移植至溫室中栽種,移植90天後,Tolumnia Snow Fairy 及Tolumnia GS248的存活率分別為100 %及5 4 . 2 %。從四個雜交組合中,依花色、花型及花香味進行選拔,從RS雜交組合中選3株、101-186雜交組合選2株及101-233雜交組合選1株。本研究建立不損壞母株的培養方法並闡明不同發育時期的花梗與類原球體誘導的關係,被選拔的後代可以用花梗或花芽培養繁殖後代。
URI: http://hdl.handle.net/11455/89169
其他識別: U0005-2307201514091900
文章公開時間: 2015-07-28
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