Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/89226
標題: 金門野百合族群與繁殖之研究
Studies of Population and Propagation of Lilium brownii on Kinmen
作者: Yue Han Yang
楊岳翰
關鍵字: 野百合, 族群, 薄層培養, 蔗糖, 植物生長調節劑
Lilium brownii, population, thin cell layer culture, sucrose, plant growth regulator
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摘要: This study is about habitat and growth cycle of Kinmen native lily in Kinmen, and collects the temperature of habitat and the precipitation by central weather bureau to discuss the relationship to the Kinmen native lily. And investigating the compatibility of Kinmen native lily. Kinmen native lilies mainly distribute over the bare stone of the path behind the granite hospital, Kinmen botanical garden and Taiwu mountain. The stem of Kinmen native lilies start development in January, flowering during April to May. And the bulbs enter dormancy after the fruits mature in July. The tepals apper different colors at different habitat. The tepals are white with green at arboreal forest, tepals are light yellow with purple lines and tepals are white with purple lines. Kinmen native lilies are self-incompatibility. Kinmen native lilies can regenerate from cutting face, adaxial side or abaxial side. The bulblets from explant are biggest and growing quickly which culture in the medium with 40 g/l sucrose. And culturing at different concentrate of plant growth regulator, the number of regenerative bulblets which culturing at 0.1 mg/l BA和0.1 mg/l NAA is most, and the diameter of regenerative bulblets is also longest (1.09 mm). The medium with NAA is good for the explants produces callus. The medium supplemented with low concentrate of NAA can produce roots directly.
本研究調查金門原生野百合在金門野外棲地、族群數量與生長週期,並收集棲地氣溫與金門水頭氣象局降雨量來探討與金門原生野百合的關係,另調查金門原生野百合的親和性。金門原生野百合主要分布於公園路花崗岩醫院後方小路裸岩、植物園以及太武山。金門原生野百合1月開始抽莖,4到5月之間開花,7月果實成熟後開始休眠。不同棲地花被片呈現不同顏色,生長在林下的花朵,外花被片為淡綠色或白色無紫紅色條紋,裸岩區或陽光照射強烈下,外花被片為白底帶有紫色條紋。金門原生野百合為自交不親和性,自交授粉著果率低,異交著果率高。金門原生野百合可從切口處、向軸面和背軸面經由薄層培養成功獲得植株。再生培養基添加40 g/l蔗糖的再生小鱗莖最大,生長速度最快。培養基添加不同植物生長調節劑中,培植體在0.1 mg/l BA和0.1 mg/l NAA濃度下,再生的小鱗莖數最多1.31個,小鱗莖平均直徑也最大(1.09 mm)。培養基添加NAA有助於癒傷組織的產生。低濃度的NAA培植體易直接產生根。
URI: http://hdl.handle.net/11455/89226
其他識別: U0005-2811201416172804
文章公開時間: 2017-08-31
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