Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/89323
標題: 台灣稻熱病菌之營養菌絲親合性
The vegetative compatibility of Magnaporthe oryzae from Taiwan
作者: Kachonsak Iamnok
甘忠誥
關鍵字: Magnaporthe oryzae
Vegetative compatibility
nit mutants
sul mutants
水稻稻熱病菌
菌絲親合性
nit 突變株
sul 突變株
引用: Altinok, H. H., Can, C. C., and Colak, H. 2013. Vegetative compatibility, pathogenicity and Virulence diversity of Fusarium oxysporum f. sp. melogenae recovered from eggplant. Journal of Phytopathology161: 651-660. Arst, H. N. 1968. Genetic analysis of the first steps of sulphate metabolism in Aspergillus nidulans. Nature 219: 268–270. Biella, S., Smith M. L., Aist, J. R., Cortesi, P., and Milgroom, M. G. 2002. Programmed cell death correlates with virus transmission in a filamentous fungus. Proceedings of the Royal Society of London, Series B 269: 2269- 2276. Busso, C., Kaneshima, E. N., Franco, F. A., and Prado, M. A. C. 2007. Genetic and molecular characterization of pathogenic isolates of Pyricularia grisea from wheat (Triticum aestivum Lam.) and triticale (x Triticosecale Wittmack) in the state of Parana, Brazil. Revista Iberoamericana de Micologia 24: 167-170. Cove, D. J. 1976. Chlorate toxicity in Aspergillus nidulans: The selection and characterization of chlorate resistance mutants. Heredity 36: 191-203. Chang, Y. C. 2001. Manual control of rice blast, Taiwan agriculture research institute, Taichung, Taiwan. Chien, C. C. 1990. The races of the rice blast pathogen. Pages 63-74. In: Tu, C. C., Tsai, T. W., Chien, C. C., Tasi, W. H., and Chang, Y. C. (Eds.) Rice Blast Disease. Special Issue of Agriculture Research No. 30, Eds., Taichung, Taiwan, 119 pp. Correll, J. C., and Leslie, J. H. 1987. Recovery of spontaneous selenate resistant mutants from Fusarium oxysporum and Fusarium moniliforme. Phytopathology 77: 1710-1717. Correll, J. C., Klittich, C. J. R., and Leslie, J .F.1987. Nitrate non utilizing mutants of Fusarium oxysporum and their use in vegetative compatibility tests. Phytopathology 72: 1640-1646. Correll, J. C., Harp, T. L., Guerber, J. C., Zeigler, R. S., Liu, B., Cartwright, R. D., and Lee, F. N. 2000.Characterization of Pyricularia grisea in the United States using independent genetic and molecular markers. Phytopathology 90: 1396-1404. Crawford, M. S., Chumley, F.G., Weaver, C.G., and Valent, B. 1986. Characterization of the heterokaryotic and vegetative diploid phases of Magnaporthe grisea. Genetics 114: 1111- 1129. Fels, I. G., and Cheldelin, V. H. 1950. Selenate inhibition studies IV. Biochemical basis of selenate toxicity in yeasts. The Journal of Biological Chemistry 185: 803-813. Jacobson, D. J., and Gordon, T. R. 1990. Further investigations of vegetative compatibility withinFusaruim oxysporum f. sp. melonis. Canadian Journal of Botany 68: 1245-1248. Javan-Nikkhah, M. 2002. Investigation on genetic diversity of populations of Magnaporthe grisea (Hebert) Barr, the rice blast fungus, using molecular, pathogenicity and vegetative compatibility characters in Guilan Province. Ph.D. Thesis. University of Tehran, Iran. GRiSP (Global Rice Science Partnership) 2013. Rice Almanac: Source Book for One of the Most Important Economic Activities on Earth, 4th Edition. International Rice Research Institute. 283 pp. George, M.L.C., Nelson, R.J., Zeigler, R.S., and Leung, H.1998. Rapid population analysis of Magnaporthe grisea by using rep-PCR and endogenous repetitive DNA sequences. Phytopathology 88: 223–229. Ghassan, J. M., and Heyam, E. A. 2010. Isolation and growth characterization of chlorate and/or bromated resistant mutants generated by spontaneous and induced forward mutation at several gene loci in Aspergillus niger. Brazilian Journal of Microbiology 41: 1099-1111. Glass, N. L., Jacobson, D. J., and Shiu, P. K. 2000. The genetics of hyphal fusion and vegetative incompatibility in filamentous ascomycete fungi. Annual Review of Genetics 34:165- 186. Harp, T. L., and Correll, J. C. 1998. Recovery and characterization of spontaneous selenate- resistant mutants of Magnaporthe grisea, the rice blast pathogen. Mycologia 90: 954–963. Hawthorne, B. T., and Rees-george, J. 1996. Use of nitrate non- utilizing mutants to study vegetative incompatibility in Fusarium solani (Nectria haematococca), especially members of mating populations I, V and VI. Mycological Research 100: 1075-1081. Hsieh, C. H., Chung, W. C., Chen, Y. N., and Chung, W. H. 2013. Phylogenetic diversity and sensitivity to MBI and Qol fungicides of Magnaporthe oryzae in Taiwan. Journal of Pesticide Science 38: 194-199. Kachroo, P., Leong, S. A., and Chattoo, B. B. 1994. Pot2, an inverted repeat transposon from the rice blast fungus Magnaporthe grisea. Molecular and General Genetics 245: 339– 348. Khush, G. S. and Jena, K. 2009. Current status and future prospects for research on blast resistance in rice (Oryza sativa L.). Pages 1-10. In: Wang, G., and Valent, B. (Eds.) Advances in Genetics, Genomics and Control of Rice Blast. Springer 430 pp. Klittich, C. J. R., and Leslie, J. F. 1989. Chlorate- resistant, nitrate utilizing (crn) mutant of Fusarium moniliforme (Gibberella fujikuroi). Journal of General Microbiology 135: 721-727. Korolev, N., and Gindin, G. 1999. Vegetative compatibility in the entomopathogen Verticillium lecanii. Mycological Research 103: 833-840. Leslie, J. F. 1993. Fungal vegetative compatibility. Annual Review of Phytopathology 31: 127–151. Leslie, J. F. and Summerell, A. B. 2006. The Fusarium Laboratory Manual. Blackeell Publishing, Ames,Iowa, USAp. 388. Leslie, J. F., and Yamashiro, C. T. 1997. Effects of the tol mutation on allelic interactions at het loci in Neurospora crassa. Genome 40: 834-40. Louws, F. J., Rademaker, J., and de Bruijn, F .1999. The three Ds of PCR-based genomic analysis of phytobacteria: Diversity, detection, and disease diagnosis. Annual Review of Phytopathology 37: 81–125. Motallebi, P. M., Javan-Nikkhah, S. M., Okhovvat, K. B. F., and Bargnil, M. 2009. Vegetative compatibility groups within Iranian populations of Magnaporthe grisea species complex from rice and some grasses. Journal of Plant Pathology 92: 469-473. Pal, K., Van diepeningen, A. D., Varga, J., Hoekstra, R. F., Dyer, P. S., and Deberts, A. J. M. 2007. Sexual and vegetative compatibility genes in the Aspergilli. Study in Mycology 59: 19- 30. Pavel, A. B., and Vasile, C. L. 2012. PyElph- a software tool for gel images analysis and phylogenetic. BMC Bioinformation 13: 9. Puhalla, J. E. 1985. Classification of Fusarium oxysporum on the basis of vegetative compatibility. Canadian Journal of Botany 63: 179-183. Ou, S. H. 1985. Rice Diseases. 2nd ed. Commonwealth. Mycological Institute, Kew, U. K. Rademaker, J. L. W. and de Bruijn, F. J. 1997. Characterization and classification of microbes by rep-PCR genomic fingerprinting and computer assisted pattern analysis. Pages 151– 171. In: Caetano-Anolle's G. and Gresshoff, P. M. (Eds) DNA Markers: Protocols, Applications, and Overviews. Wiley and Sons, Inc, New York, NY, USA. Rosenfeld, I., and Beath, O. A. 1964. Selenium: Geobotany, Biochemistry, Toxicity, and Nutrition. Academic press, New York. 411 pp. Sant' Anna, J. R., Miyamoto, C. T., Rosada, L. J., Franco, C. C. S., Kanneshima, E. N., and Castro- Prado, M. A. A. 2010. Genetic relatedness of Brazilian Collectorichum truncatum isolates assessed by vegetative compatibility groups and RAPD analysis. Biological Research 43: 51-62. Sushil, P., Derek, B., David, D., Achim, D., Samarendu, M., Scott, R. and Bill, H. 2010. Rice in the Global Economy. Strategic Research and Policy Issues for Food Security. IRRI. Taylor, J. W., Jacobson, D. J. and Fisher, M. C.1999. The evolution of asexual fungi: reproduction, speciation and classification. Annual Review of Phytopathology 37: 197-246. Treena, B., Wubetu, B., Michael, J. W. and Brenda, D. W.2009. A simple and rapid method to determine vegetative compatibility groups in fungi. Supplement to Mycologia Vol. 60 December 2009. Wang, G. 2009. Preface. In: Wang, G., and Valent, B. (Eds.) Advances in Genetics, Genomics and Control of Rice Blast. Springer 430 pp. Weeds, P. L., Beever, R. E., and Long, P. L.1998. New genetic markers for Botrytis cinerea (Botryotinia fuckeliana). Mycological Research 102: 791–800. Weissman, G. S., and Trelease, S. F. 1954. Influence of sulphate on the toxic of selenium to Aspergillus. American Journal of Botany 42: 489-495. Zhang, C. Q., and Zhou, M. G. 2006. Recovery and characterization of asexual recombinants of Magnaporthe grisea. Phytoparasitica. 34: 54-62. Zhang, C. Q., and Zhou, M. G. 2004. Recovery and biological properties of nitrate non- utilizing mutants of rice blast, Magnaporthe grisea. Rice Science 11: 214-218. Zhang, Z. Q., and Zheng, X. B. 2000. Inter- racial asexual recombination in Magnaporthe grisea. Acta Phytopathologica Sinica 30: 312-318.
摘要: Rice blast disease caused by Magnaporthe oryzae (Anamorph: Pyricularia oryzae) is a serious disease that reduces rice production worldwide. Vegetative compatibility has been used to demonstrate the genetic diversity, and thought as an important mechanism contributing genetic exchange within and between populations of ascomycetousfungi. In order to assess the vegetative compatibility of M. oryzae populations in Taiwan, agar plugs of 87 isolates were placed on minimal media (MM medium) amended with 60 g/L of potassium chlorate to recover nitrate non- utilizing (nit) mutants. The fast growing colonies, the phenotype of nit mutants on MM medium, were obtained from 17 % of agar plugs of all isolates and 71.04 % of them were real nit mutants. Phenotypic classes of nit mutants were determined by growth patterns on media which contained one of four nitrogen sources (sodium nitrate, sodium nitrite, hypoxanthine and ammonium), and were assigned as nit1, nit3, nitM or nitA. In this study, four phenotypic classes of nit mutants were recovered with unequal proportions: nitA (31.41%), nit1 (27.6%), nit3 (24.01%), and nitM (17.41%). Sulfate non-utilizing (sul) mutants were also recovered from 1176 agar plugs of 53 isolates which showed no complementation between their nit mutants. The fast growing colonies were derived from 37.5% of transferred agar plugs, and 50.34 % of them were real sul mutants. A peculiar high percentage (35.19 %) of heterokaryon self-incompatible (HSI) isolates was determined by no complementation in pairings of their nit / nit mutants and nit / sul mutants. To study the population structure of the 86 isolates, isolate 63 (a heterokaryon self-compatible isolate ) was used to be a tester isolate in order to pair with other 86 isolates with all possible combinations, resulting in four types of heterokaryon formations: negative type (21.60%), weak type (15.90%), medium type (6.81%) and strong type (55.68%). Notably, among isolates that showed strong type of heterokaryon formation withisolate 63, heterokaryon formation may not occur between each other by pairing their nit mutants. Pot2 polymorphism was used to demonstrate the population structure of 23 isolates with four types of heterokaryon formation. There was no correlation between the results of heterokaryon formation and Pot2 polymorphism. This study suggested although the pot2 polymorphism of M. oryzae isolates are diverse, they may derived from a predominate VCG group.
URI: http://hdl.handle.net/11455/89323
文章公開時間: 2016-02-03
Appears in Collections:植物病理學系

文件中的檔案:

取得全文請前往華藝線上圖書館



Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.