Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/89335
標題: 水稻徒長病菌之生物特性:殘存能力及致病之進程
Characterization of Fusarium fujikuroi, with reference to the survival and the course of disease occurrence
作者: Chieh-Yu Lin
林杰妤
關鍵字: 水稻徒長病菌
水稻徒長病
土壤殘存
選擇性培養基
營養缺陷突變株
激勃素產量
Fusarium fujikuroi
bakanae disease
survival in soil
selective medium
nit mutant
gibberellin production
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摘要: Fusarium fujikuroi是造成水稻徒長病的病原菌,常造成水稻產量嚴重損失;一般認為稻種為最主要的感染來源,造成苗期的徒長,但是在田間有的徒長病徵是在分糵期或抽穗期出現,是否這些分糵期或抽穗期才出現的病徵來自受到感染的種子,或實際上為田間帶菌的土壤所造成。本研究希望能探討土壤接種源是否與分糵及抽穗期之病徵有關,以及種子受到感染後,不同的生育期是否有不同的病徵表現。本研究為了探討徒長病菌在土壤中的殘存能力,及是否足以造成田間的植株發病,首先改良出適合偵測土壤內徒長病菌之選擇性培養基,並設計標定之突變菌株,適合專一地追蹤人為接種之徒長病菌在自然環境下的變化,因此,本研究得以長時間追蹤在不同土壤成分及環境條件下徒長病菌之殘存與消長。實驗結果發現,土壤接種源確實能在田間造成病害,但比率極低;種子接種源造成秧苗100%發病後,同樣的植株病徵可能在不同時期消失,或在病徵消失一段時間後再度呈現,或自苗期始持續呈現徒長的病徵。而徒長病菌於土壤中殘存六個月後仍然保有高濃度的接種源,足以造成病害。最後,本研究希望能了解不同徒長病菌株在激勃素產量之相對能力,以不同菌株之培養液接種矮化品系之水稻後,作為生物分析激勃素產量的方法,結果發現此方法確實具可行性。
Fusarium fujikuroi is the causal agent of bakanae disease, usually resulting in severe yield loss. It has long been considered that the infection of seed is the major cause of this disease. Symptoms occur not only on seedling stage, but also on tillering and heading stages. It is very likely that the symptoms of tillering and heading stage are from soil inoculum. The aim of this study is to investigate the correlation between soil inoculums and disease occurrence on tillering and heading stages, and to realize the disease process after seeds were infected. To evaluate the ability of survival of pathogens, modified selective medium was established, and marked nit mutants were developed. The results suggest that the soil inoculum do have the ability to cause disease, although the incidence is low. Inoculated seeds lead to 100% diseased seedlings, among which some may appear healthy in later stage (some of them may appear diseased again), some may stay diseased continuously. With regard to the production of gibberellins of different isolates, a bioassay method was designed. A dwarf mutant line of rice was used to quantify the production of gibberellins.
URI: http://hdl.handle.net/11455/89335
文章公開時間: 2017-08-30
Appears in Collections:植物病理學系

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