Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/89340
標題: 產氣內生真菌Nodulisporium sp. PDL-005之特性與培養條件暨防治貯藏病害評估
Characteristics and culture conditions of the volatile-producing endophytic fungus Nodulisporium sp. PDL-005 and evaluation for controlling postharvest diseases
作者: Che-Chia Yeh
葉哲嘉
關鍵字: 內生真菌
石蟬草
貯藏病害
生物薰蒸劑
endophytic fungi
Peperomia dindygulensis
postharvest disease
biofumigant
引用: Akutsu, K. 1995. Style of disease development by grey mould. Jouvnal of Pesticide Science 20:187-192. Alpha, C.J., Campos, M., Jacobs-Wagner, C., and Strobel, S.A. 2015. Mycofumigation by the volatile organic compound-producing fungus Muscodor albus induces bacterial cell death through DNA damage. Applied and Environmental Microbiology 81:1147-1156. Bacon, C., Porter, J., Robbins, J., and Luttrell, E. 1977. Epichloë typhina from toxic tall fescue grasses. Applied and Environmental Microbiology 34:576-581. Banerjee, D., Pandey, A., Jana, M., and Strobel, G. 2014. Muscodor albus MOW12 an endophyte of Piper nigrum L. (Piperaceae) collected from north east India produces volatile antimicrobials. Indian Journal of Microbiology 54:27-32. Barkai-Golan, R. 2001a. Biological Control. Pages 221-251 in: Postharvest diseases of fruits and vegetables, R. Barkai-Golan, ed. Elsevier, Amsterdam. Barkai-Golan, R. 2001b. Physical Means. Pages 189-220 in: Postharvest diseases of fruits and vegetables, R. Barkai-Golan, ed. Elsevier, Amsterdam. Barkai-Golan, R. 2001c. Chemical Control. Pages 147-188 in: Postharvest diseases of fruits and vegetables, R. Barkai-Golan, ed. Elsevier, Amsterdam. Batty, A., Dixon, K., Brundrett, M., and Sivasithamparam, K. 2001. Constraints to symbiotic germination of terrestrial orchid seed in a mediterranean bushland. New Phytologist 152:511-520. Bills, G.F. 1996. Isolation and analysis of endophytic fungal communities from woody plants. Pages 31-65 in: Endophytic Fungi in Grasses and Woody Plants: Systematics, Ecology, and Evolution, S.C. Redlin and L.M. Carris, eds. APS Press, St. Paul, Minnesota, U.S.A. Caccioni, D.R., Guizzardi, M., Biondi, D.M., Renda, A., and Ruberto, G. 1998. Relationship between volatile components of citrus fruit essential oils and antimicrobial action on Penicillium digitatum and Penicillium italicum. International Journal of Food Microbiology 43:73-79. Chandrasekhar, S., Satyanarayana, K., Pramada, P., Raghavan, P., and Gupta, T. 2003. Review processing, properties and applications of reactive silica from rice husk—an overview. Journal of Materials Science 38:3159-3168. Chen, J.H., Tsai, H.F., and Lin, Y.W. 2004. Evaluation of the suitablity of three analysis methods for determining organic matter contents in fertilizers. Taiwanese Journal of Agricultural Chemistry and Food Science 42:116-124. Chen, J.W. 2009a. Fungal flora in of Phalaenopsis and the study of Fusarium solani associated with Phalaenopsis yellowing leaf disease. Taichung: Master thesis of National Chung Hsing University, pp. 85. Chen, L., Zhou, Y., and Dong, J.X. 2007a. Chemical constituents of Peperomia dindygulensis. Chinese Traditional and Herbal Drugs 38:491-493. Chen, L., Zhou, Y., and Dong, J. 2007b. Three new flavonoid glycosides from Peperomia dindygulensis. Acta Pharmaceutica Sinica 42:183-186. Chen, L., Zhou, Y.L., Zhou, Y., and Dong, J.X. 2008. Hydrophobic constituents of Peperomia dindygulensis Miq. Natural Product Research and Development 20:275-277. Chen, P.S. 2009b. Control of orange green mold with gas-producing bacteria and analysis of their major gas components suppressing the pathogen. Taichung: Master thesis of National Chung Hsing University, pp. 63. Chitwood, D.J. 2003. Research on plant-parasitic nematode biology conducted by the United States Department of Agriculture-Agricultural Research Service. Pest Management Science 59:748-753. Coates, L., and Johnson, G. 1997. Postharvest diseases of fruit and vegetables. Pages 533-548 in: Plant pathogens and plant diseases, J.F. Brown and H. Ogle, eds. Rockvale Publications. Corcuff, R., Mercier, J., Tweddell, R., and Arul, J. 2011. Effect of water activity on the production of volatile organic compounds by Muscodor albus and their effect on three pathogens in stored potato. Fungal Biology 115:220-227. Cowan, M.M. 1999. Plant products as antimicrobial agents. Clinical Microbiology reviews 12:564-582. Du Toit, P.J., Olivier, S.P., and van Biljon, P.L. 1984. Sugar cane bagasse as a possible source of fermentable carbohydrates. I. Characterization of bagasse with regard to monosaccharide, hemicellulose, and amino acid composition. Biotechnology and Bioengineering 26:1071-1078. Evans, H.C., Holmes, K.A., and Thomas, S.E. 2003. Endophytes and mycoparasites associated with an indigenous forest tree, Theobroma gileri, in Ecuador and a preliminary assessment of their potential as biocontrol agents of cocoa diseases. Mycological Progress 2:149-160. Ezra, D., and Strobel, G.A. 2003. Effect of substrate on the bioactivity of volatile antimicrobials produced by Muscodor albus. Plant Science 165:1229-1238. Ezra, D., Hess, W.M., and Strobel, G.A. 2004. New endophytic isolates of Muscodor albus, a volatile-antibiotic-producing fungus. Microbiology 150:4023-4031. Faith, N.G., Garcia, G., Skebba, V.P., Gandhi, N.R., and Czuprynski, C.J. 2015. Use of a commercial mixture of volatile compounds from the fungus Muscodor to inhibit Salmonella in ground turkey and beef. Food Control 47:628-633. Gabler, F.M., Fassel, R., Mercier, J., and Smilanick, J.L. 2006. Influence of temperature, inoculation interval, and dosage on biofumigation with Muscodor albus to control postharvest gray mold on grapes. Plant Disease 90:1019-1025. Gao, L., Bao, W.Y., Zhang, T.W., Shan, H.W., and MA, S. 2013. Effect of water C∶N ratio on the growth, antagonism and C∶N ratio of Bacillus, lactic acid bacteria and vibrio. Periodical of Ocean University of China 43:34-40. Guo, N., Jiang, S.T., Li, X.J., and Pan, L.J. 2013. Extraction of wheat bran protein and amino acid composition analysis. Journal of Hefei University of Technology 36:224-227. Hirsch, G., and Braun, U. 1992. Communities of parasitic microfungi. Pages 225-250 in: Fungi in vegetation science, W. Winterhoff, ed. kluwer academic, Dordrecht, Netherlands. Hoveland, C.S. 1993. Importance and economic significance of the Acremonium endophytes to performance of animals and grass plant. Agriculture, Ecosystems and Environment 44:3-12. Hsieh, T.F. 2014. Current trends on research and development of botanical fungicides or plant protection products derived from plant materials. Proceedings of the Conference on the Development of Agricultural Biological Materials Industry:117-140. Hung, Y.N. 2013. Comparison between cinnamon and Ceylon Cinnamon in the compositions, anti-inflammatory and anti- Propionibacterium acnes , anti- Pityrosporum ovale abilities of essential oils. Tainan: Master Thesis of Chia-Nan University of Pharmacy and Science, pp. 116. Jafarpour, M., Jalalizand, A., and Eghbalsaied, S. 2011. High fiber media as the most efficient substrates for Pleurotus florida culture. Archives of Biological Sciences 63:889-895. Kent, C.R., Ortiz-Bermudez, P., Giles, S.S., and Hull, C.M. 2008. Formulation of a defined V8 medium for induction of sexual development of Cryptococcus neoformans. Applied and Environmental Microbiology 74:6248-6253. Lü, X., Xia, W.L., Liu, T.C., Sakoda, A., and Suzuki, M. 2001. A new process ro resource rice hull. Transactions of the CSAE 17:132-135. Lacey, L.A., Horton, D.R., and Jones, D.C. 2008. The effect of temperature and duration of exposure of potato tuber moth (Lepidoptera: Gelechiidae) in infested tubers to the biofumigant fungus Muscodor albus. Journal of Invertebrate Pathology 97:159-164. Lee, S.O., Kim, H.Y., Choi, G.J., Lee, H.B., Jang, K.S., Choi, Y.H., and Kim, J.C. 2009. Mycofumigation with Oxyporus latemarginatus EF069 for control of postharvest apple decay and Rhizoctonia root rot on moth orchid. Journal of Applied Microbiology 106:1213-1219. Li, G., Lin, M., Yang, G., Yang, Y., and Qin, X. 2012a. Determination of Peperomin B in Peperomia dindygulensis Miq. by HPLC. Chinese Journal of Pharmaceuticals 5:344-346. Li, Q., Ning, P., Zheng, L., Huang, J., Li, G., and Hsiang, T. 2010. Fumigant activity of volatiles of Streptomyces globisporus JK-1 against Penicillium italicum on Citrus microcarpa. Postharvest Biology and Technology 58:157-165. Li, Q., Ning, P., Zheng, L., Huang, J., Li, G., and Hsiang, T. 2012b. Effects of volatile substances of Streptomyces globisporus JK-1 on control of Botrytis cinerea on tomato fruit. Biological Control 61:113-120. Lin, S.Y. 2013. Wheat bran as the substrate for cellulase production by Aspergillus niger under solid-state fermentation. Taipei: Master Thesis of Tatung University, pp. 60. Lomer, C., Bateman, R., Johnson, D., Langewald, J., and Thomas, M. 2001. Biological control of locusts and grasshoppers. Annual review of entomology 46:667-702. Luh, B.S. 1991. Rice hulls. Pages 269-294 in: Rice, volume 2: Utilization, B.S. Luh, ed. Springer Science & Business Media. Malinowski, D.P., and Belesky, D.P. 2000. Adaptations of endophyte-infected cool-season grasses to environmental stresses: mechanisms of drought and mineral stress tolerance. Crop Science 40:923-940. Mamputu, M., and Buhr, R. 1995. Effect of substituting sesame meal for soybean meal on layer and broiler performance. Poultry Science 74:672-684. Mari, M., Di Francesco, A., and Bertolini, P. 2014. Control of fruit postharvest diseases: old issues and innovative approaches. Stewart Postharvest Review 10:1-4. Matheron, M., Porchas, M., and Bigelow, D. 2006. Factors affecting the development of wood Rot on lemon trees infected with Antrodia sinuosa, Coniophora eremophila, and a Nodulisporium sp. Plant Disease 90:554-558. Mends, M.T., Yu, E., Strobel, G.A., Riyaz-Ul-Hassan, S., Booth, E., Geary, B., Sears, J., Taatjes, C.A., and Hadi, M.Z. 2012. An endophytic Nodulisporium sp. producing volatile organic compounds having bioactivity and fuel potential. Journal of Petroleum and Environmental Biotechnology. Mercier, J., and Jiménez, J.I. 2004. Control of fungal decay of apples and peaches by the biofumigant fungus Muscodor albus. Postharvest Biology and Technology 31:1-8. Mercier, J., and Smilanick, J.L. 2005. Control of green mold and sour rot of stored lemon by biofumigation with Muscodor albus. Biological Control 32:401-407. Mercier, J., and Jimenez, J.I. 2007. Potential of the volatile-producing fungus Muscodor albus for control of building molds. Canadian Journal of Microbiology 53:404-410. Mercier, J., Jiménez-Santamaría, J.I., and Tamez-Guerra, P. 2007. Development of the volatile-producing fungus Muscodor albus Worapong, Strobel, and Hess as a novel antimicrobial biofumigant. Revista Mexicana de Fitopatología 25:173-179. Meunchang, S., Panichsakpatana, S., and Weaver, R.W. 2005. Co-composting of filter cake and bagasse; by-products from a sugar mill. Bioresource Technology 96:437-442. Minerdi, D., Bossi, S., Gullino, M.L., and Garibaldi, A. 2009. Volatile organic compounds: a potential direct long-distance mechanism for antagonistic action of Fusarium oxysporumstrain MSA 35. Environmental Microbiology 11:844-854. Mitchell, A., Strobel, G., Hess, W., Vargas, P., and Ezra, D. 2008. Muscodor crispans, a novel endophyte from Ananas ananassoides in the Bolivian Amazon. Fungal Diversity 31:37-43. Nielsen, K.F., Holm, G., Uttrup, L.P., and Nielsen, P.A. 2004. Mould growth on building materials under low water activities. Influence of humidity and temperature on fungal growth and secondary metabolism. International Biodeterioration and Biodegradation 54:325-336. Parameswaran, B. 2009. Sugarcane bagasse. Pages 239-252 in: Biotechnology for Agro-Industrial Residues Utilisation, P. Singh nee' Nigam and A. Pandey, eds. Springer Netherlands. Park, M.S., Ahn, J., Choi, G.J., Choi, Y.H., Jang, K.S., and Kim, J.C. 2010. Potential of the volatile-producing fungus Nodulisporium sp. CF016 for the control of postharvest diseases of apple. The Plant Pathology Journal 26:253-259. European Parliament and Council of the European Union. 2004. Directive 2004/42/CE of the european parliament and of the council of 21 April 2004. Official Journal of the European Union L143:87-96. Peng, G.X., Zhang, Y.J., Yang, X.Y., Wang, Z.K., and Pei, Y. 2000. Relationship of the production of subtilisin-like protease and total extracellular protease by Beauveria bassiana with its virulence to Bombyx mori. Chinese Journal of Biological Control 16:61-64. Porras-Alfaro, A., and Bayman, P. 2011. Hidden fungi, emergent properties: endophytes and microbiomes. Phytopathology 49:291. Redman, R.S., Sheehan, K.B., Stout, R.G., Rodriguez, R.J., and Henson, J.M. 2002. Thermotolerance generated by plant/fungal symbiosis. Science 298:1581-1581. Revankar, S.G., Patterson, J.E., Sutton, D.A., Pullen, R., and Rinaldi, M.G. 2002. Disseminated phaeohyphomycosis: review of an emerging mycosis. Clinical Infectious Diseases 34:467-476. Riga, E., Lacey, L.A., and Guerra, N. 2008. Muscodor albus, a potential biocontrol agent against plant-parasitic nematodes of economically important vegetable crops in Washington State, USA. Biological Control 45:380-385. Riyaz-Ul-Hassan, S. 2013. An endophytic Nodulisporium sp. from central America producing volatile organic compounds with both biological and fuel potential. Journal of Microbiology and Biotechnology 23:29-35. Sasan, R.K., and Bidochka, M.J. 2012. The insect-pathogenic fungus Metarhizium robertsii (Clavicipitaceae) is also an endophyte that stimulates plant root development. American journal of botany 99:101-107. Schardl, C.L., and Phillips, T.D. 1997. Protective grass endophytes: where are they from and where are they going? Plant Disease 81:430-438. Schulz, B., Boyle, C., Draeger, S., Römmert, A.K., and Krohn, K. 2002. Endophytic fungi: a source of novel biologically active secondary metabolites. Mycological Research 106:996-1004. Sharma, A., Diwevidi, V., Singh, S., Pawar, K.K., Jerman, M., Singh, L., Singh, S., and Srivastawa, D. 2013. Biological control and its important in agriculture. International Journal of Biotechnology and Bioengineering Research 4:175-180. Sharma, N., and Tripathi, A. 2006. Fungitoxicity of the essential oil of Citrus sinensis on post-harvest pathogens. World Journal of Microbiology and Biotechnology 22:587-593. Sharma, R.R., Singh, D., and Singh, R. 2009. Biological control of postharvest diseases of fruits and vegetables by microbial antagonists: A review. Biological Control 50:205-221. Shu, Y., Guo, S.X., Zhang, D.M., and Wang, C.L. 2005. Studies on active components from endophytic fungi. Chinese Traditional and Herbal Drugs 36:772-776. Sikora, R.A., Pocasangre, L., zum Felde, A., Niere, B., Vu, T.T., and Dababat, A. 2008. Mutualistic endophytic fungi and in-planta suppressiveness to plant parasitic nematodes. Biological Control 46:15-23. Stein, E., Molitor, A., Kogel, K.-H., and Waller, F. 2008. Systemic resistance in Arabidopsis conferred by the mycorrhizal fungus Piriformospora indica requires jasmonic acid signaling and the cytoplasmic function of NPR1. Plant and Cell Physiology 49:1747-1751. Stierle, A., Strobel, G., and Stierle, D. 1993. Taxol and taxane production by Taxomyces andreanae, an endophytic fungus of pacific yew. Science 260:214-216. Stinson, A., Zidack, N., Strobel, G., and Jacobsen, B. 2003. Mycofumigation with Muscodor albus and Muscodor roseus for control of seedling diseases of sugar beet and Verticillium wilt of eggplant. Plant Disease 87:1349-1354. Strobel, G.A. 2006. Muscodor albus and its biological promise. Journal of Industrial Microbiology and Biotechnology 33:514-522. Strobel, G.A., Dirkse, E., Sears, J., and Markworth, C. 2001. Volatile antimicrobials from Muscodor albus, a novel endophytic fungus. Microbiology 147:2943-2950. Strobel, G.A., Miller, R.V., Martinez-Miller, C., Condron, M.M., Teplow, D.B., and Hess, W. 1999. Cryptocandin, a potent antimycotic from the endophytic fungus Cryptosporiopsis cf. quercina. Microbiology 145:1919-1926. Suwannarach, N., Kumla, J., Bussaban, B., Nuangmek, W., Matsui, K., and Lumyong, S. 2013. Biofumigation with the endophytic fungus Nodulisporium spp. CMU-UPE34 to control postharvest decay of citrus fruit. Crop Protection 45:63-70. Tan, R.X., and Zou, W.X. 2001. Endophytes: a rich source of functional metabolites. Natural Product Reports 18:448-459. Tripathi, P., and Dubey, N.K. 2004. Exploitation of natural products as an alternative strategy to control postharvest fungal rotting of fruit and vegetables. Postharvest Biology and Technology 32:235-245. Umabala, P., Lakshmi, V., Murthy, A.R., Prasad, V.S., Sundaram, C., and Beguin, H. 2001. Isolation of a Nodulisporium species from a case of cerebral phaeohyphomycosis. Journal of Clinical Microbiology 39:4213-4218. Wang, R.J., Cheng, S.H., Liou, Y.T., Chang, C.M., Chang, H.Y., and Chang, S.J. 2010. Standardization of evaluated parameters of strawberry cultivars by principle components analysis techniques. Journal of the Agricultural Association of Taiwan 11:105-120. White, T.J., Bruns, T., Lee, S., and Taylor, J.W. 1990. Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. Pages 315-322 in: PCR protocols: a guide to methods and applications, M.A. Innis, D.H. Gelfand, J.J. Sninsky, and T.J. White, eds. Academic Press, New York. Wu, J.l., Li, N., Hasegawa, T., Sakai, J.i., Mitsui, T., Ogura, H., Kataoka, T., Oka, S., Kiuchi, M., and Tomida, A. 2006. Bioactive Secolignans from Peperomia dindygulensis. Journal of natural products 69:790-794. Wu, S.Y. 2012. Diversity of fungal endophytes from Cinnamomum kanehirai and the analyses of bioactive materials of the Colletotrichum sp. isolate CKL005. Taichung: Mater Thesis of National Chung Hsing University. Wu, S.Y., Chung, W.C., Huang, J.W., Ishii, H., and Chung, W.H. 2009. Identification and fungicidal sensitivity of the fungus Venturia sp. the causal agent of pear scab in Taiwan. Plant Pathology Bulletin 18:135-143, pp. 84. Xu, F.L. 2010. Effect of NO fumigation on quality of post-harvest tomatoes. Fujian Journal of Agricultural Sciences 25:72-76. Yang, C.P., Chen, H.B., Wu, W.J., and Gu, A.G. 2005. Diversity of plant endophytic fungi secondary metabolites and their potential applications. Acta Agriculturae Boreali-occidentalis Sinica 14:126-132. Yang, C.W. 2006. Effect of tea seed pomace on control cabbage seedling damping-off caused by Rhizoctonia solani AG-4 and indentification for its major ingredient of antifungal activity. Taichung: Mater Thesis of National Chung Hsing University, pp. 49. Yuan, Z.L., Chen, Y.C., Xu, B.G., and Zhang, C.L. 2012. Current perspectives on the volatile-producing fungal endophytes. Critical reviews in biotechnology 32: 363-373. Zaferanloo, B., Mahon, P.J., and Palombo, E.A. 2012. Endophytes from medicinal plants as novel sources of bioactive compounds. Pages 355-411 in: Medicinal plants: biodiversity and drugs, M.K. Rai, G.A. Cordell, J.L. Martinez, M. Marinoff, and L. Rastrelli, eds. CRC Press. Zeiger, E., and Tice, R. 1997. Review of toxicological literature. North Carolina: Integrated Laboratory Systems, pp. 15. Zhu, W., Lin, M., Yang, G., Wang, Q., and Yang, Y. 2011. Two novel polyketides from Peperomia dindygulensis. Chinese Traditional Herbal Drugs 42:420-422.
摘要: 植物內生真菌(endophytic fungi)為棲息於健康植物組織中的一群真菌,可產生和寄主植物產物相似的活性物質,其中可產生有機揮發性化合物(volatile organic compounds, VOCs)者可稱為產氣內生真菌(volatile-producing endophytic fungi)。本研究自石禪草(Peperomia dindygulensis)葉片組織中分離出7株具產氣活性之內生真菌,以Nodulisporium sp. PDL-005最佳。培養於potato sucrose agar(PSA)平板三天之PDL-005菌株所產生之活性揮發物質,能抑制多種貯藏病原真菌之菌絲生長,對草莓灰黴病菌(Botrytis cinerea)等病原真菌之抑制率可達100.0%,而對柳橙綠黴病菌(Penicillium digitatum)之抑制率介於88.2-99.4%,雖具防治潛力但抑制效果變異度較高,為篩選可穩定表現PDL-005菌株拮抗能力之基質,以察氏培養基(Czapek's medium)添加不同碳氮素源進行測試。結果得知,添加單一供試碳氮素源之察氏培養基,無法促進PDL-005菌株產生拮抗綠黴病菌的氣體,然將PDL-005菌株培養於potato dextrose agar(PDA)、malt extract agar(MEA)或oat meal dextrose agar(OMDA)等培養基時,可提高該菌株之拮抗能力,其中以培養在MEA培養基時之抑菌效果最佳,抑制率可達100%並具有致死效果。進一步測試PDL-005菌株培養於芝麻粕、苦茶粕、大豆粕、小麥粒、小麥麩、燕麥、米糠、粗糠及蔗渣等9種基質的生長情形,並評估PDL-005菌株抑制綠黴菌生長的效果,結果得知,培養於蔗渣之PDL-005菌株生物量雖低,但抑制效果最好(90.6-100.0%)且具致死效果,而培養於小麥麩上之PDL-005菌株生物量與最高之芝麻粕組別無顯著差異,且對柳橙綠黴病菌的抑制率次高(約86.4%)。為了提高培養於蔗渣之PDL-005的生物量,將小麥麩以1:3、1:6及1:9比例(w / w)混合蔗渣,測試對PDL-005菌株生長與活性影響。顯示隨著小麥麩比例越高,PDL-005菌株生物量增加越多,然抑制率卻逐漸下滑,並失去對柳橙綠黴病菌之致死作用。續將PDL-005菌株培養於蔗渣且經吹乾磨碎後製成PDL-005粉粒,並將該粉粒以2:3比例(w / w)與蔗渣混合後放入中藥袋製成PDL-005製劑,於加水活化1天後,實際測試對貯藏病害之防治效果。得知6 L之容器內,20 g之PDL-005製劑對草莓灰黴病之防治率可達100%;而30 g之PDL-005製劑對柳橙綠黴病之防治率亦可達100%。利用GC-MS分析PDL-005菌株所產生之主要氣體成份,得知隨培養基質與培養天數不同,PDL-005菌株所產生之揮發性物質種類與含量亦有所變化,其中主要活性物質為2-ethyl-2-hexenal與2,4-dimethyl-1,3-cyclopentanedione。 本研究所開發之PDL-005製劑,具有防治貯藏病害之潛力。未來可進一步優化PDL-005製劑的配方與使用方式,評估該製劑使用上的風險,並擴大測試對於其他病蟲害的防治效果;亦可人工合成PDL-005菌株之主要活性物質,作為生物源薰蒸劑施用。
Endophytic fungi inhabiting healthy plants can produce some phytochemicals with the same characteristic of their hosts. Some endophytic fungi that can produce volatile organic compounds(VOCs)are called volatile-producing endophytic fungi. In this study, seven volatile-producing endophytic fungi were obtained from the leaf of Peperomia dindygulensis, among all endophytic fungi, the bioactivity of Nodulisporium sp. PDL-005 was best. VOCs produced by 3-day-old PDL-005 isolate cultured on PSA could inhibit several postharvest fungal pathogens mycelial growth, for example, it can completely inhibit several fungal pathogens such as Botrytis cinerea, while to Penicillium digitatum, the ratio of inhibition is from 88.2% to 99.4%. The efficacy of controlling P. digitatum was obvious but varied a lot. For screening the nutrients to stabilize antifungal ability, the PDL-005 isolate was cultured on the Czapek's medium which different carbon or nitrogen sources were added to. The results indicated that the single carbon or nitrogen source could not enhance the ability against tested postharvest pathogens. However, the activity of PDL-005 isolate could be enhanced while it was cultured on potato dextrose agar(PDA)、malt extract agar(MEA)and oat meal dextrose agar(OMDA).Among all medium, the inhibition effect of PDL-005 isolate cultured on MEA was the best(100%)with a killing effect. The nine substrates, including sesame meal, tea seed pomace, soybean meal, wheat grain, wheat bran, oatmeal, rice bran, rice hulls and bagasse were tested in efficiency of enhancing mycelial growth of PDL-005 and the inhibition of P. digitatum. The result showed that the biomass of PDL-005 cultured on bagasse was low, while the inhibition effect against P. digitatum was the best(90.6-100.0%)and had a killing effect. Furthermore, the biomass of PDL-005 cultured on wheat bran was no difference against the highest biomass group-sesame meal and the inhibition rate of PDL-005 isolate against P. digitatum was the second highest(about 86.4%)among all substances. In order to enhance the biomass of PDL-005 cultured on bagasse, the effect was examed that bagasse was mixed with wheat bran in the ratio of 1:3, 1:6 and 1:9 on biomass and inhibition ability of PDL-005 against P. digitatum was examed. The result showed that the biomass of PDL-005 increased more in substrate containing higher proportion of wheat bran, but the inhibition rate of PDL-005 isolate against P. digitatum gradually decreased and lost the killing effect. Next, the PDL-005 grain was made from PDL-005 isolate cultured on bagasse under the process of dried-and-grinded. Furthermore, the PDL-005 grain was mixed with bagasse in the ratio of 2:3 and put into a medicine bag as the PDL-005 product. After rehydration of PDL-005 product for one day, the control value of PDL-005 product against postharvest diseases was examed. The result showed that the control value of 20 g PDL-005 product against gray mold of strawberry, the control value of 30 g PDL-005 product against green mold of orange were 100% in 6L food storage box. The major compound of VOCs produced by PDL-005 isolate was analyzed by GC-MS. The type and content of VOCs produced by PDL-005 isloate changed with culture day and different medium or substances, and the major active compound produced by PDL-005 isolate were 2-ethyl-2-hexenal and 2,4-dimethyl-1,3-cyclopentanedione. The PDL-005 product developed in this study showed good potential on controlling postharvest diseases. In the future, the formulation and usage of the PDL-005 product can be further optimized. Furthermore, the risk of using PDL-005 should be assessed and the control effect of PDL-005 against other pest should be further examed, or the major active compound produced by PDL-005 could be artificially generated as a biological source fumigant, instead.
URI: http://hdl.handle.net/11455/89340
文章公開時間: 2018-08-28
Appears in Collections:植物病理學系

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