Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/89358
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dc.contributor黃振文zh_TW
dc.contributor.authorPo-Hao Chiuen_US
dc.contributor.author邱柏皓zh_TW
dc.contributor.other植物病理學系所zh_TW
dc.date2014zh_TW
dc.date.accessioned2015-12-07T07:25:37Z-
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dc.identifier.urihttp://hdl.handle.net/11455/89358-
dc.description.abstractPseudoperonospora cubensis引起之露菌病(downy mildew)是胡蘆科作物重要的病害之一,雖然化學藥劑可以有效防治胡瓜露菌病,但抗藥菌系的出現與化學農藥對於環境與人類的負面效應,發展替代的防治策略成為我們努力探討的目標。本研究主要目的在於建立胡瓜露菌病菌的接種平台,進而評估非農藥資材防治胡瓜露菌病的可行性。將P. cubensis孢囊點滴接種於胡瓜切離葉,於20-24℃第四天可引起水浸狀病徵;此外將孢囊(5 sporangia/10 μl)點滴接種於胡瓜切離葉片後,在20℃每天光照12小時的生長箱中,保濕培養7天後,發現葉齡14 和21天之胡瓜切離葉的發病率最高可達90%以上,且可產生較多量的新孢囊。將接種過的切離葉於不同時間切取葉片圓盤,移置於飽和chloral hydrate(250 g/100 ml)中脫色後,再以lacto-phenol cotton blue進行染色,隨後於光學顯微鏡下觀察,發現露菌游走孢子於接種後8小時內可在氣孔附近形成靜止子;在第16小時前則已發芽侵入葉組織內部。篩選胡瓜露菌病的防治資材試驗中,發現250 ppm的自製油酸鉀(potassium oleate),可完全抑制孢囊的萌發;至於市售之油酸鉀濃度需要500 ppm才可有同等的抑制效果。利用油酸鉀分別將葵花油(SFO)、花生油(PNO)、大豆油(SBO)及苦茶油(CAO)乳化後,以胡瓜切離葉片接種平台評估,發現以油酸鉀乳化葵花油、花生油和大豆油之製劑,其250倍稀釋液可顯著減少60%露菌病的罹病率。在溫室中測試植物油乳化製劑的防病效果,發現油酸鉀乳化葵花油製劑及花生油製劑之250倍稀釋液,於接種病原菌前24小時施用於胡瓜植株上可顯著降低露菌病罹病度達20%左右。測試不同無機物質對露菌孢囊萌發的影響試驗中,發現10 ppm二氧化氯可完全抑制露菌孢囊的萌發。此外,將Bacillus mycoides BM02發酵液添加不同濃度幾丁聚醣(CN)後,進而處理胡瓜露菌病菌,發現CN的濃度可顯著影響露菌病菌孢囊的發芽率,當CN濃度在0.5%以上,其500倍稀釋液可抑制89%的孢囊萌發;若CN濃度達2%時,即可完全抑制孢囊的萌發。進一步,在溫室以BMCN2(在BM02發酵液中添加2% CN)、PO-A(市售之油酸鉀)及PO-B(自製之油酸鉀)分別噴佈於胡瓜植株,其中250倍和500倍的BMCN2稀釋液可顯著減少胡瓜露菌病的發生,並減少17%和18%的罹病度;此外,於接種前施用PO-A和PO-B也可有效減少胡瓜露菌病的罹病度11%和20%。在接種前將BMCN2製劑澆灌處理於胡瓜植株根部,結果隨著澆灌次數增加,胡瓜植株發生露菌病的罹病度會隨之下降,其中BMCN2 100倍稀釋液可降低罹病度達36.7%。綜合上述成果,証明植物油乳化製劑SFOE、PNOE和SBOE及微生物製劑BMCN2可推薦給農友作為防治胡瓜露菌病的非農藥資材。zh_TW
dc.description.tableofcontents中文摘要 ........................................................................................................................ i 英文摘要 ................................................................................................................... iii 目次............................................................................................................................. vi 表次?引..................................................................................................................... ix 圖次?引...................................................................................................................... x 前言............................................................................................................................. 1 材?與方法.................................................................................................................. 6 一、 供試植物........................................................................................................ 6 二、 供試菌株?源、分?和觀察........................................................................ 6 三、 供試菌株繁殖、培養及保存........................................................................ 6 (一) 以???培養菌種........................................................................................ 6 (二) 以?圓盤培養菌種........................................................................................ 7 (三) 菌株保存........................................................................................................ 7 四、 接種源製備與接種方法................................................................................ 8 (一) 噴佈接種法.................................................................................................... 8 (二) 點滴接種法.................................................................................................... 8 五、 病害發生條件分析........................................................................................ 8 (一) 溫?對P. cubensis PC-001 孢囊釋放游走孢子之影響 ............................... 8 (二) P. cubensis PC-001 孢囊釋放游走孢子之?? ............................................ 8 (三) 溫?對P. cubensis PC-001 引起胡瓜????菌病之影響 ....................... 9 (四) 病原菌接種源濃?對胡瓜?菌病發生之影響............................................ 9 (五) ?齡對胡瓜?菌病發生之影響.................................................................... 9 (?) 胡瓜?菌病菌侵染過程................................................................................ 9 (七) 胡瓜?菌病之溫室接種試驗...................................................................... 10 ?、 非生物性資材防治胡瓜?菌病之效果評估.............................................. 10 (一) 油酸鉀製備.................................................................................................. 10 (二) 植物油之乳化法.......................................................................................... 10 (三) ?同濃?油酸鉀對P. cubensis PC-001 孢囊釋放游走孢子之影響 ......... 11 (四) 植物油乳化劑防治胡瓜?菌病之效果...................................................... 11 (五) 無機物質對P. cubensis PC-001 孢囊釋放游走孢子之影響 ..................... 12 七、 生物性資材防治胡瓜?菌病之效果評估.................................................. 12 (一) ?同濃?之幾丁聚醣添加於蕈?芽孢桿菌發酵液對P. cubensis PC-001 孢囊釋放游走孢子之影響.................................................................................. 12 (二) BMCN2 防治胡瓜?菌病之效果 ............................................................... 13 八、 溫室試驗...................................................................................................... 13 (一) ?同施用時間對於PO-A、PO-B 和BMCN2 防治胡瓜?菌病之影響 . 13 (二) ?同濃?BMCN2 澆灌於胡瓜植株防治胡瓜?菌病之效果評估 .......... 14 (三) ?同施用時間對於植物油乳化製劑防治胡瓜?菌病之影響.................. 14 結果......................................................................................................................... 15 一、 ???培養胡瓜?菌.................................................................................. 15 二、 胡瓜?菌形態觀察...................................................................................... 15 三、 胡瓜?菌病發生條件分析.......................................................................... 15 (一) 溫?對P. cubensis PC-001 孢囊釋放游走孢子之影響 ............................. 15 (二) P. cubensis PC-001 孢囊釋放游走孢子釋放?? ...................................... 15 (三) 溫?對P. cubensis PC-001 於胡瓜???引起?菌病之影響 ................. 16 (四) 接種源濃?對胡瓜?菌病發生之影響...................................................... 16 (五) ?齡對於胡瓜?菌病發生之影響.............................................................. 16 (?) 胡瓜?菌病侵染過程.................................................................................. 16 (七) 胡瓜?菌病之溫室接種試驗...................................................................... 17 四、 非生物性資材防治胡瓜?菌病之效果...................................................... 17 (一) ?同濃?油酸鉀對P. cubensis PC-001 孢囊釋放游走孢子之影響 ......... 17 (二) 植物油乳化劑防治胡瓜?菌病之效果...................................................... 17 (三) 無機物質對P. cubensis PC-001 孢囊釋放游走孢子之影響 ..................... 18 五、 生物性資材防治胡瓜?菌病的效果.......................................................... 18 (一) ?同濃?之幾丁聚醣添加於蕈?芽孢桿菌發酵液對P. cubensis PC-001 孢囊釋放游走孢子之影響.................................................................................. 18 (二) BMCN2 防治胡瓜?菌病之效果 ............................................................... 18 ?、 溫室試驗...................................................................................................... 19 (一) ?同施用時間對PO-A、PO-B 和BMCN2 防治胡瓜?菌病之效果 ..... 19 (二) ?同濃?BMCN2 澆灌於胡瓜植株防治胡瓜?菌病之效果評估 .......... 19 (三) ?同施用時間對於植物油乳化製劑防治胡瓜?菌病之影響.................. 19 討?........................................................................................................................... 21 ?考文獻................................................................................................................... 25zh_TW
dc.language.isozh_TWzh_TW
dc.rights同意授權瀏覽/列印電子全文服務,2017-08-31起公開。zh_TW
dc.subjectPseudoperonospora cubensiszh_TW
dc.subject胡瓜?菌病zh_TW
dc.subject油酸鉀(potassium oleate)zh_TW
dc.subjectBacillus mycoides BM02zh_TW
dc.subject幾丁聚醣zh_TW
dc.subject乳化植物油zh_TW
dc.subjectPseudoperonspora cubensisen_US
dc.subjectcucumber downy mildewen_US
dc.subjectpotassium oleateen_US
dc.subjectemusifed plant oilsen_US
dc.subjectBacillus mycoides BM02en_US
dc.subjectchitosanen_US
dc.title非農藥資材防治胡瓜?菌病之研究試驗zh_TW
dc.titleControl experiments of cucumber downy mildew with non-pesticide materialsen_US
dc.typeThesis and Dissertationen_US
dc.date.paperformatopenaccess2017-08-31zh_TW
dc.date.openaccess2017-08-31-
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