Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/90244
標題: 乳酸菌微膠囊化最適化的探討
Studies on the optimization of the microencapsulation of lactic acid bacteria
作者: 黃子芸
Tzu-Yun Huang
關鍵字: Lactobacillus acidophilus
Lactobacillus salivarius
microencapsulation
extrusion
response surface methodology (RSM)
alginate
pectin
chitosan
trehalose
Lactobacillus acidophilus
Lactobacillus salivarius
微膠囊化
擠壓法
反應曲面法
褐藻酸鈉
果膠
幾丁聚醣
海藻糖
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摘要: Latic acid bacteria could offer lots of health effects to humankind has been extensively studied, and Lactobacillus acidophilus is one of famous probiotics which plays an important role in dairy fermentation. By intake those fermented food contained live L. acidophilus can get many health benefits has been confirmed. In addition, Lactobacillus salivarius has gained attention in recent years as a promising probiotic species, has the potential with the characteristic of anti-Helicobacter pylori and well immune regulation. However, these two latic acid bacterias have been maintained a number of viable cells which has shown to be efficacious in human gastrointestinal tract. Therefore, this study used microencapsulation to entrap latic acid bacteria. A three-variable and three-level design method, analyzed by response surface methodology, was used to study the effects of sodium alginate concentration, pectin concentration, and CaCl2 concentration on the survival of L. acidophilus, and to determine the optimum conditions. Results showed that 1.5% sodium alginate, 1.5% pectin, and 0.3M CaCl2 combining with 1% chitosan had the highest survival rate, then evaluate the health effects of L. acidophilus miceocapsule prepared by this optimum conditions. This study also used extrusion to prepare microencapsulated Lactobacillus salivarius with different treatments, such as concentration, fermentation and adding trehalose. The purpose of using those different treatments to prepare probiotic microcapsules is to enhance its application. This would provide traditional probiotic microcapsule with more variability and applicability. The optimum conditions were determined by efficiency of L. salivarius and to evaluate its health effects. The results showed that the microcapsules CE1-2, CE1-0 and ES-2 have higher efficiency, and the microcapsule containing concentrated entrapped and surface L. salivarius fermented in MRS broth supplemented with 1% CaCl2(CE1-0) and without trehalose has the highest viability when treated with simulated digestive juices. Besides, the results of L. acidophilus miceocapsule prepared by optimum conditions and the CE1-0 L. salivarius microcapsule including β-galactosidase activity, acidification power test, and protein analysis tested with simulated digestive juices all showed significantly improved compared with free bacteria. Indicated these two microcapsules could improve the tolerance to simulated gastrointestinal conditions and maintain good viability, activity and metabolic activity.
乳酸菌所能對人體帶來的健康益處已被廣泛研究,而Lactobacillus acidophilus即為著名之益生菌(probiotics)之一,其在乳製品發酵的加工地位已是不可或缺,這些含有L. acidophilus活菌的益生發酵食品,在攝入人體後能提供許多的益生功效已被廣泛的研究與證實。另外Lactobacillus salivarius則為最近十年來漸受到重視之益生菌,極具益生菌潛力,能有效抑制胃幽門螺旋桿菌,且對於免疫的調節能力相當強。然而此兩株乳酸菌在到達腸道作用部位前必須維持一定的菌數與活性才得以提供宿主健康益處,因此本研究利用微膠囊化技術將乳酸菌加以包覆,並以反應曲面法針對不同褐藻酸鈉、果膠與氯化鈣溶液濃度三個因子對L. acidophilus微膠囊之交互作用進行探討,並找出可獲得最高菌體存活率之最適操作條件。結果顯示配合1%幾丁聚醣的作用下,以褐藻酸鈉濃度1.5%、果膠濃度1.5%、氯化鈣濃度0.3M條件所製備之微膠囊有最佳之菌體存活率,再對此操作條件所得之微膠囊進行各種性狀測定。此外再製備依不同菌液處理及發酵方式並添加海藻糖所得之七種型態L. salivarius微膠囊,使傳統僅將菌體包埋於微膠囊中之方式有更多的變化性與應用性,並自各不同製備條件中取最佳菌體包埋率之操作條件進行各種性狀測定,發現CE1-2、CE1-0與ES-2具有極佳之包埋率,而在經過體外模擬消化液的作用下,則以不添加海藻糖之包含濃縮包埋菌體與表面增殖菌體(以含1%氯化鈣MRS broth發酵)之CE1-0微膠囊具有最佳的腸胃道耐受性。此外,此L. salivarius CE1-0微膠囊與上述最適條件所得之L. acidophilus 微膠囊在模擬消化液連續作用後之益生功效評估中,產酸能力、β-半乳糖苷酶活性、蛋白質分析或掃描式電子顯微鏡觀察等,與游離態菌體相較皆有較佳的表現,顯示此兩種微膠囊能在模擬腸胃道的環境中提供有效保護,保持菌體的活性與代謝能力。
URI: http://hdl.handle.net/11455/90244
文章公開時間: 10000-01-01
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