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標題: 酵素催化合成綠原酸酯之最適化探討
Optimization of lipase-catalyzed synthesis of chlorogenate ester
作者: Tsung-Han Lin
關鍵字: Keywords: Lipase
response surface methodology
chlorogenic acid
enzymatic synthesis
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摘要: 綠原酸(chlorogenic acid)可被當為有機相中之抗氧化劑,但綠原酸在有機相的溶解度較差,因此提高綠原酸的親脂性為本文研究的方向。 本實驗分成兩部分 第一部分以綠原酸當成酸和辛醇(ocatanol)來進行酯化反,應生成綠原酸酯,實驗探討反應時間(12h ~ 36h)、酵素用量(10 ~ 50mg)及超音波功率(90 ~ 150W)對綠原酸轉化率之影響,結果顯示反應時間、酵素用量對於綠原酸的轉化率皆有顯著影響。以三階層三變數之 Box-Behnken design (BBD)及反應曲面法探討綠原酸酯化之轉化率,結果顯示最適化合成反應條件為反應時間12h,酵素用量 50mg 及功率 120W,此條件下所得到綠原酸轉化率為 95.3%。 第二部分以綠原酸當醇並和辛酸(octanoic acid)進行反應並以固定化酵素Novozyme 435 催化合成綠原酸酯,在使用溶劑 2M2B 情況下進行合成。實驗選取三個反應因素因子莫爾數比 (綠原酸比辛酸)、酵素用量、反應時間。其中莫爾數比及酵素用量皆會對轉換率產生極大影響。再以三階層三變數之 Box-Behnken design (BBD)及反應曲面法探討綠原酸酯化之轉化率,分析對合成綠原酸酯的影響。實驗結果最適化條件為:莫爾數比 1:300、酵素用量 140mg 及反應天數為 2 天時,可得到綠原酸酯轉化率為 36.5%。 關鍵字:脂解酵素、反應曲面法、綠原酸、最適化、酯化反應、酵素合成
Chlorogenic acid is usually used as antioxidants in the organic phase; however, its low solubility causes a problem when applied in industry. To increase the lipophilic property of chlorogenic acid is the primary aim in this study. This study is divided into two parts. In the first part, chlorogenic acid is used as an acid and octanol was employed as alcohol in the esterification reaction to produce chlorogenic acid ester. Factors such as reaction time (12-36 h), enzyme amount (10-50 mg) and power (90-150 W) were studied. The results show that the reaction time and the enzyme amount have the most significant effect on the conversion of chlorogenic acid. The 3-level-3-factor Box-Behnken design (BBD) was applied to optimize the reaction conversion. The optimal reaction conditions are as follows: reaction time 12 h, enzyme amount 50mg, and ultrasound power of 120W. Under this condition, an esterification conversion of 95.3% can be reached. In part two, chlorogenic acid is employed as an alcohol and octanoic acid was used as the acid in the esterification reaction to produce chlorogenic acid ester. 2-methyl-2-butanol (2M2B) is chosen as the solvent. Box-Behnken design was adopted to evaluate the effect of three different parameters, i.e., molar ratio (1:100-1:300), enzyme amount (20-140mg) and reaction time (1-3day). The results show that the molar ratio and the enzyme amount have the most significant effect on chlorogenic acid conversion. By ridge max analysis, the optimal reaction conditions were found as follows: molar ratio 1:348.5, enzyme amount 159.6 mg, and reaction time 2.19 days. Under this condition, the highest conversion of 36.8%±0.8 can be obtained. Keywords: Lipase、response surface methodology、chlorogenic acid、optimal、esterification、enzymatic synthesis
其他識別: U0005-2805201516440800
文章公開時間: 10000-01-01
Appears in Collections:化學工程學系所



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