Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/91513
標題: 以微電極陣列作為生物感測器之應用
Microelectrode arrays for biosensor application
作者: Chien-Nung Kuo
郭建儂
關鍵字: microelectrode
biosensor
hydrogen peroxide
微電極
生物感測器
過氧化氫
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摘要: 微電極本身因具有較小的電極尺寸、快速充電電雙層(double-layer)、降低歐姆電壓iR、增加訊號雜訊比(signal-to-noise ratio)和高質傳速率等特性,可用於電分析量測。本實驗微電極由西班牙國家高等科學研究委員會研究團隊所提供,指叉電極陣列使用產生-收集模式(generator-collector mode)比起產生電極可以放大電流訊號達到9.1倍且收集效率為0.97,將辣根過氧化酵素固定在指叉電極陣列上,加入過氧化氫後利用指叉電極陣列產生-收集模式來放大電流訊號,其線性範圍5μM-500μM,偵測極限1.22μM,靈敏度為0.6238 A / (M cm2),由干擾物測試證明指叉電極陣列感測器可避免抗壞血酸與尿酸的干擾。
Microelectrodes are electrode with characteristic dimensions on the micrometer scale. Faster double-layer charging, reduced ohmic loss, increased signal-to-noise ratio and high mass-transport rates have allowed them to revolutionize electroanalytical measurements. Hydrogen peroxide biosensors are fabricated by immobilization of horseradish peroxidase (HRP) onto the surface of interdigitated microband electrodes. The interdigitated microband electrode shows an amplification factor of ~9.1 with a collection efficiency of 0.97, demonstrating a high efficiency in generator-collector experiments. The resulting hydrogen peroxide biosensor exhibits a sensitivity of 0.6238 A M-1 cm-2, a detection limit of 1.22 μM, and a linear range of 5-500 μM. The performance of the proposed biosensor was evaluated with potential interferences such as ascorbic acid and uric acid.
URI: http://hdl.handle.net/11455/91513
其他識別: U0005-0907201415102501
文章公開時間: 2017-07-11
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