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標題: 添加中草藥萃取液於牛樟芝液態發酵生產血管收縮酵素抑制劑之研究
Effect of addition of Chinese herbal extracts on ACEI production by Antrodia cinnamomea submerged cultivation
作者: Yi-Jing Li
關鍵字: Antrodia cinnamomea
angiotensin converting enzyme inhibitor (ACEI)
Gastrodia elata
Mesona chinensis
Scutellaria baicalensis
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摘要: 牛樟芝(Antrodia cinnamomea),為台灣特有種真菌,文獻研究顯示其富含多醣體、三萜類、倍半萜類化合物和類固醇物質,在抗腫瘤、抗氧化、消炎及抗高血壓等都有顯著的效果。而中草藥天麻、仙草和黃芩具有防止血栓形成和降血壓等生理活性功能,常用於中藥師治療高血壓之藥方中。為建立中草藥(天麻、黃芩和仙草)的萃取液與牛樟芝在降血壓功效上是否具有協同或加乘之功效,進行以中草藥萃取液做為牛樟芝醱酵之培養原料,探討中草藥(天麻、黃芩和仙草)萃取液對提昇樟芝ACEI的影響。 在探討中草藥的影響之前,首先探討四種不同碳源之影響。以glucose對牛樟芝生長與ACEI最活性最高,總抑制相當於2220.0 ng captopril/L之濃度;接下來以五種不同氮源添加與不同天數培養試驗,以yeast extract 為氮源培養時,在第八天有最高產量,其值為9.44gDW/L;而以peptone對牛樟芝生長與ACEI效果最好,在第九天有最高抑制效果,總抑制相當於4929.5 ng captopril/L。 找出適當的碳氮源之條件之後,加入中草藥萃取物為培養基作為探討變因。菌量生長方面,以添加10g/L天麻萃取液產出之菌量最多,為13.59gDW/L;添加10g/L仙草萃取液培養之樟芝擁有最高的總抑制效果,抑制效果相當於8202.1 ng captopril/L,相當於控制組的3.2倍;而添加不同濃度的黃芩萃取液會使樟芝的菌量和抑制活性都降低。因此,可得知天麻有助於樟芝之生長,而仙草有提升樟芝的ACEI的效果。
Antrodia cinnamomea is an endemic species of fungi in Taiwan. Previous reports indicated that fruiting body of A. cinnamomea is characterized by varied physiological active compounds such as polysaccharrides, triterpenes, sesquiterpenes, and steroids et al, which proved to be effective for anti-oxidation, anti-tumor,anti-inflammatory, hepatoprotective and antihypertension treatments. Traditional Chinese herbs, such as Gastrodia elata, Mesona chinensis and Scutellaria baicalensis have been reported having the function of reducing blood pressure. In this project, the submerged culture of A.cinnamomea was carried out associated with the addition of these three Chinese herbal extracts, in order to elevate the ACEI activity of A.cinnamomea. In carbon source test, glucose gave had the highest biomass and ACE inhibition. In the nitrogen source test, yeast extract yeilded the highest biomass at eighth-day and peptone as nitrogen source produced the highest ACE inhibition, which was equal to 4929.5 ng captopril/L. The addition of Chinese herbal extracts to A.cinnamomea cultivation was conducted. The highest biomass had 13.59 gDW/L by adding 10g/L of Gastrodia elata ectracts. The highest ACE inhibition was equal to 8202.1 ng captopril/L by adding 10g/L of Mesona chinensis extracts, which was 3.2 times higher than the control test. However, the biomass and ACE inhibition were both decreased by adding different concentration of Scutellaria baicalensis extracts.
其他識別: U0005-1408201514574900
文章公開時間: 10000-01-01
Appears in Collections:化學工程學系所



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