Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/92157
標題: 春蘭和蝴蝶蘭莖頂與苦瓜花粉超低溫冷凍保存流程之探討
Investigation on Protocols of Cryopreservation in Shoot Tips of Cymbidium goeringii (Rchb. f.) Rchb. f. and Phalaenopsis and Pollen of Momordica charantia L. 'F-948'
作者: Kuan-Fu Chen
陳冠輔
關鍵字: 
NO
引用: 林怡如、李穎宏、陳正敏。 (2008) 。 南瓜和苦瓜抗氧化能力探討。 高雄區農 業改良場年報。pp.72。/ 林曜松、呂光洋、黃生、劉小如、金恆鑣、趙榮台、邵廣昭、徐源泰、謝伯娟、 黃永慶。 (2002) 。 國家生物多樣性研究之規劃報告。國立台灣大學生物多樣 性研究中心。pp.1-41。/ 周鎮。 (1986) 。 臺灣蘭圖鑑:地生蘭篇。臺中 臺灣。PP. 7-86。 /陳文輝、邱明森、位國慶、楊秀蘭。 (1994) 。 蘭花栽培 - 蝴蝶蘭。臺中農業 試驗所。pp. 220-229。 /蔡月夏。 (1994) 。 蘭花栽培-國蘭。臺中農業試驗所。pp.259-264。 /蕭吉雄 (1995) 。 蔬菜種原之利用 作物種原保育技術研習會專刊 pp.56-64。。。。 /王昭月、曾夢蛟。 (2004) 。 植物體胚細胞、莖頂與花粉冷凍保存之研究。植 物種苗。6,1-12。 /王源龍。 (2004) 。 番木瓜莖頂之液態氮超低溫保存方法及傷害之探討。國立 中興大學生命科學系 博士論文。 /全中和、楊素絲。 (2013) 。 苦瓜新品種`花蓮 5 號'之育成。 花蓮區農業改 良場研究彙報。31, 13-19。 /江徽之。 (2012) 。 臺灣白花蝴蝶蘭莖頂玻璃化法超低溫冷凍保存流程之探討。 國立中興大學生命科學系 碩士論文。 /艾鵬飛、羅正榮。 (2004) 。 柿品種`禪寺丸'花粉超低溫保存研究。 華中農 業大學學報。23, 563-565。 /李坤紘。 (2008) 。 台灣藥用植物食茱萸與牛樟玻璃化法超低溫冷凍保存前處 理流程之探討。國立中興大學生命科學系 碩士論文。 /李定雄。 (2009) 。 蝴蝶蘭大白花選育及遺傳特性分析。國立嘉義大學園藝學 系 碩士論文。 /招彥明。 (2013) 。 大葉佛來明豆頂芽及芥藍花粉超低溫冷凍保存流程之探討。 國立中興大學生命科學系 碩士論文。 /林怡君。 (2002) 。 台農 57、台農 68 品種甘藷超低溫冷凍保存前處理流程之 探討。 國立中興大學生命科學系 碩士論文。 /林經剴。 (2009) 。 臺灣原生藥用植物石薺薴和六角草玻璃化法超低溫冷凍保 存流程之探討。國立中興大學生命科學系 碩士論文。 /林維熙。 (2008a) 。 台灣原生藥用植物-高氏柴胡玻璃質化法超低溫冷凍保存流 程之探討。國立中興大學生命科學系 碩士論文。 /林蓉萱。 (2008b) 。 苦瓜對肺癌細胞之抗腫瘤作用。 慈濟大學生命科學研究 所學位論文。 /邱智聖。 (2011) 。 青花菜和臺灣蝴蝶蘭花粉乾燥法超低溫冷凍保存流程之探 討。 國立中興大學生命科學系 碩士論文。 /洪永倫。 (2009) 。 大蒜玻璃化法超低溫冷凍保存流程之探討。國立中興大學 生命科學系 碩士論文。 /洪惠娟、郭珮琪、魏芳明。 (2010) 。 臺灣國蘭產業及外銷作業調查。 臺中區 農業改良場研究彙報。109, 29-40。 /胡馨分。 (2006) 。 北蕉玻璃化法超低溫冷凍保存前處理流程之探討。國立中 興大學生命科學系 碩士論文。 /張心怡。 (2013) 。 結球白菜和不結球白菜花粉超低溫冷凍保存流程之探討。 國立中興大學生命科學系 碩士論文。 /張正桓。 (2006) 。 苦瓜花粉型態、花粉活力、授粉及果實生長之研究。國立 中興大學園藝學系 碩士論文。 /張正桓、 宋妤。 (2006) 。 苦瓜花粉儲藏溫度、期間與授粉方式對著果之影響。植物種苗。8, 17-30。 /張有明、鄭櫻慧、許華欣、黃鵬林。 (2000) 。 苦瓜果實構造及發育之研究 Ⅱ 苦瓜胚、種子和果實的發育。 中華農業研究。49, 49-60。 /郭宏遠、宋妤。 (2007) 。 花粉保存與利用。 植物種苗。9, 48-58。 /陳文輝。 (2002) 。 蝴蝶蘭的品種改良。 科學發展。351, 32-39。 /陳岱陽。 (2008) 。 台灣四種中草藥 - 地筍、台灣黃岑、石香薷、生毛將軍玻 璃質化法超低溫冷凍保存處理流程之探討。國立中興大學生命科學系 碩士論 文。 /陳玟君。 (2006a) 。 台農 13 號鳳梨玻璃化法超低溫冷凍保存前處理流程之探 討。國立中興大學生命科學系 碩士論文。 /陳玟潔。 (2012) 。 沉香玻璃化法超低溫冷凍保存流程之探討。國立中興大學 生命科學系 碩士論文。 /陳婉屏。 (2003) 。 台南育六號樹薯超低溫冷凍保存前處理流程之探討。國立 中興大學生命科學系 碩士論文。 /陳裕星、張莉欣。 (2004) 。 臺灣原生蕙蘭屬植物遺傳資源之分類與生育特性。 臺中區農業改良場研究彙報。82, 51-60。 /陳瓊均。 (2006b) 。 巨峰與蜜紅葡萄玻璃化法超低溫冷凍保存前處理流程之探 討。國立中興大學生命科學系 碩士論文。 /彭燕玲。 (2012) 。 臺灣原生植物青脆枝玻璃化法超低溫冷凍保存流程之探討。 國立中興大學生命科學系 碩士論文。 /黃乃芸、陳阿發。 (2009) 。 苦瓜. [Bitter Gourd]. 健康世界。283, 98-99。 /黃秀琪。 (2007a) 。 市售不同苦瓜對抗發炎反應之影響。 亞洲大學生活應用 科學學系 碩士班學位論文。 /黃坤陽。 (2007b) 。 草莓玻璃化法超低溫冷凍保存流程之探討。國立中興大學 生命科學系 碩士論文。 /黃敬涵。 (2012) 。 臺灣原生植物牛樟玻璃化法超低溫冷凍保存流程之探討。 國立中興大學生命科學系 碩士論文。 /董英、張慧慧。 (2008) 。 苦瓜多糖降血糖活性成分的研究。 營養學報。30, 54-56。 /詹金鳳。 (2011) 。 臺灣原生藥用植物艾納香玻璃化法超低溫冷凍保存流程之 探討。國立中興大學生命科學系 碩士論文。 /廖松淵、陳清義。 (1992) 。 大豆滲透調節與耐旱性之關係。 中華農學會報。 158, 19-28。 /廖郁欣。 (2013) 。 腎葉山螞蝗腋芽及油菜花粉超低溫冷凍保存流程之探討。 國立中興大學生命科學系 碩士論文。 /劉淑芬。 (2005) 。 台農 16 號鳳梨玻璃化法超低溫冷凍保存前處理流程之探 討。國立中興大學生命科學系 碩士論文。 /蔡叔芬。 (2004) 。 臺農 25、臺農 64 與臺農 66 品種甘藷玻璃化法超低溫冷 凍保存前處理流程之探討。國立中興大學生命科學系 碩士論文。 /戴元熏。 (2012) 。 花椰菜和青花菜花粉乾燥法超低溫冷凍保存流程之探討。 國立中興大學生命科學系 碩士論文。 /羅文陽。 (2007) 。 番木瓜玻璃質化法超低溫冷凍保存流程之探討。國立中興 大學生命科學系 碩士論文。 /羅智明。 (1998) 。 不同前處理對原生報歲蘭超低溫冷凍保存傷害之探討。國 立中興大學植物學系 碩士論文。 /蘇上傑。 (2013) 。 臺灣原生藥用植物擬紫蘇草莖頂玻璃化法超低溫冷凍保存 流程之探討。國立中興大學生命科學系 碩士論文。 /Engelmann, F., and Dussert, S. (2013). Cryopreservation Conservation of Tropical Plant Species. Springer, pp. 107-119. /Lambardi, M., and De Carlo, A. (2003). Application of tissue culture to the germplasm conservation of temperate broad-leaf trees. Micropropagation of Woody Trees and Fruits. Springer, pp. 815-840. /Reed, B.M., and Uchendu, E. (2008). Controlled rate cooling. Plant Cryopreservation: A Practical Guide. Springer, pp. 77-92. /Salisbury, F.B., and Ross,C.W. (1992). Plant physiology and plant cells. In Plant Physiology,F.B. Salibury,and C.W. Ross, (eds.). Belmont,CA: Wadsworth, Inc, pp. 3-26. /Shivanna, K.R., and Johri, B.M. (1985). The Angiosperm Pollen : Structure and Function. Wiley Eastern, pp. 1-374 /Slavík, B. (1974). Water in cells and tissues. In: Slavik B. (eds.). Methods of Studying Plant Water Relations, pp.1-20. /Steer, M.W., and Steer, J.M. (1989). Tansley review no. 16. Pollen tube tip growth. New Phytologist, pp. 323-358. /Yoshida, S., Forno, J. H., Cook, H., and Gornez, K. A. (1976). Determination of sugar and starch in plant tissue. Laboratory Manual of Physiological Studies of Rice. The International Rice Research Institute, Philipines, pp.46-49. /Assy-Bah, B., and Engelmann, F. (1992). Cryopreservation of mature embryos of coconut (Cocos nucifera L.) and subsequent regeneration of plantlets. CryoLetters 13, 117-126. /Bachiri, Y., Gazeau, C., Hansz, J., Morisset, C., and Dereuddre, J. (1995). Successful cryopreservation of suspension cells by encapsulation-dehydration. Plant Cell Tiss. Organ Cult. 43, 241-248. /Bajaj, Y.P.S. (1979). Technology and prospects of cryopreservation of germplasm. Euphytica 28, 267-285. /Benelli, C., De Carlo, A., and Engelmann, F. (2013). Recent advances in the cryopreservation of shoot-derived germplasm of economically important fruit trees of /Actinidia, Diospyros, Malus, Olea, Prunus, Pyrus and Vitis. Biotechnol. Adv. 31, 175-185. /Berjak, P., Pammenter, N.W., and Wesley-Smith, J. (2011). The effects of various parameters during processing for cryopreservation on the ultrastructure and viability of recalcitrant zygotic embryos of Amaryllis belladonna. Protoplasma 249, 155-169. /Bian, H.-W., Wang, J.-H., Lin, W.-Q., Han, N., and Zhu, M.-Y. (2002). Accumulation of soluble sugars, heat-stable proteins and dehydrins in cryopreservation of protocorm-like bodies of Dendrobium candidum by the air-drying method. J. Plant Physiol. 159, 1139-1145. /Bradford, M. (1976). A rapid and sensitive method for quantification of microgram quantities of protein utilizing the principle of protein dye binding. Anal. Biochem. 72, 248-254. /Brewbaker, J.L., and Kwack, B.H. (1963). The essential role of calcium ion in pollen germination and pollen tube growth. Am. J. Bot. 50, 859-865. /Bukhov, N.G., Popova, E.V., and Popov, A.S. (2006). Photochemical activities of two photosystems in Bratonia orchid protocorms cryopreserved by vitrification method. Rus. J. Plant Physiol. 53, 793-799. /Chen, X.L., Li, J.H., Xin, X., Zhang, Z.E., Xin, P. P., and Lu, X.X. (2011). Cryopreservation of in vitro-grown apical meristems of Lilium by droplet-vitrification. S. Afr. J. Bot. 77, 397-403. /Dumet, D., Engelmann, F., Chabrillange, N., and Duval, Y. (1993). Cryopreservation of oil palm (Elaeis guineensis Jacq.) somatic embryos involving a desiccation step. Plant Cell Rep. 12, 352-355. /Dussert, S., Chabrillange, N., Rocquelin, G., Engelmann, F., Lopez, M., and Hamon, S. (2001). Tolerance of coffee (Coffea spp.) seeds to ultralow temperature exposure in relation to calorimetric properties of tissue water, lipid composition, and cooling procedure. Physiol. Plant. 112, 495-504. /Engelmann, F. (2011). Use of biotechnologies for the conservation of plant biodiversity. In Vitro Cell Dev. Biol.-Plant. 47, 5-16. /Fatima, S., Mujib, A., Nasim, S.A., and Siddiqui, Z.H. (2009). Cryopreservation of embryogenic cell suspensions of Catharanthus roseus L.(G) Don. Plant Cell Tiss. Organ Cult. 98, 1-9. /Ferri, A., Bellini, E., Padula, G., and Giordani, E. (2008). Viability and in vitro germinability of pollen grains of olive cultivars and advanced selections obtained in Italy. Adv. Hortic. Sci. 22, 1000-1007. /Flachsland, E., Terada, G., Scocchi, A., Rey, H., Mroginski, L., and Engelmann, F. (2006). Cryopreservation of seeds and in vitro-cultured protocorms of Oncidium bifolium Sims. (Orchidaceae) by encapsulation-dehydration. CryoLetters 27, 235-242. /Fukai, S., and Oe, M. (1990). Morphological observations of chrysanthemum shoot tips cultured after cryoprotection and freezing. J. Jpn. Soc. Hortic. Sci. 59, 383-387. /Gamez-Pastrana, R., Gonzalez-Arnao, M.T., Martinez-Ocampo, Y., and Engelmann, F. (2011). Thermal events in calcium alginate beads during encapsulation dehydration and encapsulation-vitrification protocols. Acta Hortic. 908,47-54. /Gogoi, K., Kumaria, S., and Tandon, P. (2013). Cryopreservation of Cymbidium eburneum Lindl. and C. hookerianum Rchb. f., two threatened and vulnerable orchids via encapsulation–dehydration. In Vitro Cell Dev. Bio.- Plant.49, 248-254. /Grapin, A., Dorion, N., Verdeil, J.L., and Escoute, J. (2007). Histo-cytological changes in Pelargonium apices during the cryopreservation process: effect of the osmotic agent chosen for the preculture step. Acta Hortic. 760, 195-201. /Guarnieri, M., Speranza, A., Nepi, M., Artese, D., and Pacini, E. (2006). Ripe pollen carbohydrate changes in Trachycarpus fortunei: the effect of relative humidity. Sex Plant Reprod. 19, 117-124. /Halmagyi, A., and Pinker, I. (2006). Plant regeneration from Rosa shoot tips cryopreserved by a combined droplet vitrification method. Plant Cell Tiss. Organ Cult. 84, 145-153. /Kim, H.-H., Popova, E., No, N.-Y., Baek, H.-J., Kim, C.-K., Cho, E.-G., and Engelmann, F. (2011). Application of alternative loading solutions to garlic and chrysanthemum in droplet-vitrification procedures. Acta Hortic. 908, 173-180. /Kim, H.H., Cho, E.G., Baek, H.J., Kim, C.Y., Keller, E.R., and Engelmann, F. (2004). Cryopreservation of garlic shoot tips by vitrification: effects of dehydration, rewarming, unloading and regrowth conditions. CryoLetters 25, 59-70. /Kim, H.H., Lee, Y.G., Shin, D.J., Ko, H.C., Gwag, J.G., Cho, E.G., and Engelmann, F. (2009). Development of alternative plant vitrification solutions in droplet-vitrification procedures. CryoLetters 30, 320-334. /Kulus, D., and Zalewska, M. (2014). Cryopreservation as a tool used in long-term storage of ornamental species–A review. Sci. Hortic. 168, 88-107. /Matsumoto, T., Sakai, A., and Yamada, K. (1994). Cryopreservation of in vitro-grown apical meristems of wasabi (Wasabia japonica) by vitrification and subsequent high plant regeneration. Plant Cell Rep. 13, 442-446. /Mayer, E., and Gottsberger, G. (2000). Pollen viability in the genus Silene (Caryophyllaceae) and its evaluation by means of different test procedures. Flora. 195, 349-353. /Mazur, P. (1984). Freezing of living cells: mechanisms and implications. Am. J. Physiol. Cell Physiol. 247, 125-142. /Mikuła, A., Fiuk, A., and Rybczyński, J.J. (2005). Induction, maintenance and preservation of embryogenic competence of Gentiana cruciata L. cultures. Acta Biol. 47, 227-236. /Pawlowska, B., and Bach, A. (2011). Cryopreservation by encapsulation-dehydration of in vitro grown shoot buds of Rosa 'New Dawn'. Acta Hortic. 908, 303-308. /Pennisi, E. (2010). Tending the global garden. Science. 329, 1274-1277. /Reed, B.M., Sarasan, V., Kane, M., Bunn, E., and Pence, V.C. (2011). Biodiversity conservation and conservation biotechnology tools. In Vitro Cell Dev. Biol.-Plant. 47, 1-4. /Rogers, S.M.D. (2003). Tissue culture and wetland establishment of the freshwater monocots Carex, Juncus, Scirpus, and Typha. In Vitro Cell Dev. Biol.-Plant. 39, 1-5. /Rosell, P., Herrero, M., and Galan Sauco, V. (1999). Pollen germination of cherimoya (Annona cherimola Mill.). In vivo characterization and optimization of in vitro germination. Sci. Horti. 81, 251-265. /Sakai, A., Kobayashi, S., and Oiyama, I. (1990). Cryopreservation of nucellar cells of navel orange (Citrus sinensis Osb. var. brasiliensis Tanaka) by vitrification. Plant Cell Rep. 9, 30-33. /Sakai, A., and Engelmann, F. (2007). Vitrification, encapsulation-vitrification and droplet-vitrification: a review. CryoLetters 28, 151-172. /Sekizawa, K., Yamamoto, S., Rafique, T., Fukui, K., and Niino, T. (2011). Cryopreservation of in vitro-grown shoot tips of carnation (Dianthus caryophyllus L.) by vitrification method using aluminium cryo-plates. Plant Biotechnol. 28, 401-405. /Suzuki, M., Ishikawa, M., Okuda, H., Noda, K., Kishimoto, T., Nakamura, T., and Akihama, T. (2006). Physiological changes in gentian axillary buds during two-step preculturing with sucrose that conferred high levels of tolerance to desiccation and cryopreservation. Ann. Bot. 97, 1073-1081. /Swarts, N.D., and Dixon, K.W. (2009). Terrestrial orchid conservation in the age of extinction. Ann. Bot. 104, 543-556. /Takagi, H., Thinh, N., Islam, O.M., Senboku, T., and Sakai, A. (1997). Cryopreservation of in vitro-grown shoot tips of taro (Colocasia esculenta (L.) Schott) by vitrification. 1. Investigation of basic conditions of the vitrification procedure. Plant Cell Rep. 16, 594-599. /Uragami, A., Lucas, M.O., Ralambosoa, J., Renard, M.,and Dereuaddre, J. (1993). Cryopreservation of microscope embryos of oilseed rape (Brassica napus L.) by dehydration in air with or without alginate encapsulation. CryoLetters 14, 83-90. /Wang, Q., Mawassi, M., Sahar, N., Li, P., Violeta, C.T., Gafny, R.,and Perl, A. (2004). Cryopreservation of grapevine (Vitis spp.) embryogenic cell suspensions by encapsulation–vitrification. Plant Cell Tiss. Organ Cul. 77, 267-275. /Xu, X.B., Cai, Z.G., Gu, Q.Q., and Zhang, Q.M. (2006). Cell ultrastructure of kiwifruit ( Actinidia chinensis) shoot tips during cryopreservation. Agr. Sci. China. 5, 587-590. /Zalewska, M., and Kulus, D. (2013). Cryopreservation of in vitro-grown shoot tips of chrysanthemum by encapsulation-dehydration. Folia. Hortic. 25, 133-140.
摘要: This study used in vitro grown of Cymbidium goeringii (Rchb. f.) Rchb. f. and Phalaenopsis Sogo Yukidian 'V3' and pollen of Momordica charantia L. 'F-948' three kind of material for cryopreservation. Explant different, the appropriate cryopreservation methods will not be the same. Cymbidium goeringii (Rchb. f.) Rchb. f. and Phalaenopsis Sogo Yukidian 'V3'shoot tips were cryopreserved by vitrification. Furthermore, the shoot tips were explored for the effect of preculture and cryprotectant and analyzed for physiological state after preculture treatments. Otherwise, pollen of Momordica charantia L. 'F-948' was cryopreseved by desiccation dehydration approach and explored the relationship among water content, in vitro and in vivo germination test. Plantlets of Cymbidium goeringii (Rchb. f.) Rchb. f. were cultured on liquid proliferation medium for 3 month, transplanted to 0.2 M sucrose medium for 60 days, excised 2 - 2.5mm shoot tips, treated with LS for 60 min and dehydrated with PVS2 for 180 min. The survival rate could reach to 62.5 - 83.3%. Plantlets of Phalaenopsis Sogo Yukidian 'V3' were cultured on growth medium for 3 month, transplanted to 0.3 M sucrose medium for 5 days, excised 2 - 2.5mm shoot tips, treated with LS for 120 min and dehydrated with PVS2 for 120 min. The survival rate could reach to 79.2 - 91.7%. Physiological analysis of this two material shoot tips shows that preculture decreased realative water content, water potential and osmotic potential, and increased soluble sugar and protein concentration. This result indicated that the physiological state of explants was regulated with osmotic adjustment during preculture treatment, which influenced tolerance of dehydrated and frozen, hence increasing survival rate. The in vitro germination test of Momordica charantia L. 'F-948'on 10% sucrose B&K medium have best germination rate. Dehydrated the anthers by silica desiccant, lastly immerged into liquid nitrogen. Pollen dehydrated for 120 - 180 min reached the better germination after cryopreservation. The germination rate could reach to 21.4 - 27.9%, at the time, the water content is between 34.7 - 56.2%. The in vivo test were use dehydrated 140 min for pollination. No difference between fruit, seed number, F1 phenotype with control. It means that pollen still can maintain the vitality and fertilizing capacity. This experiment advise the protocol for Cymbidium goeringii (Rchb. f.) Rchb. f. and Phalaenopsis Sogo Yukidian 'V3' cryopreservation by vitrification and the favorable water content for pollen cryopreservation of Momordica charantia L. 'F-948' by desiccation dehydration. It can be served as a reference for germplasm conservation by cryopreservation.
本試驗以春蘭 (Cymbidium goeringii (Rchb. f.) Rchb. f.) 與蝴蝶蘭大白花品 種'V3' (Phalaenopsis Sogo Yukidian 'V3') 無菌組織培養苗及苦瓜`F-948' (Momordica charantia L. 'F-948') 花粉三種材料進行超低溫冷凍保存,培植體不 同,所適合的超低溫冷凍保存方法亦不同。春蘭與蝴蝶蘭莖頂以玻璃化法進行保 存,探討蔗糖預培養處理及冷凍保護劑對存活率之影響,並以生理分析探討預培 養對此兩種材料之影響。苦瓜花粉以矽膠脫水乾燥法進行保存,探討花粉含水率 與超低溫冷凍保存後之離體萌發率和活體萌發率相互關係。 春蘭以液態培養方式培植 3 個月之芽體,移至 0.2 M 蔗糖培養基預培養 60 天,切取 2 - 2.5 mm 莖頂,以冷凍保護劑 LS 處理 60 min,PVS2 處理 180 min, 存活率可達 62.5 - 83.3% 。蝴蝶蘭大白花品種'V3'以培植於生長培養基 3 個月之 植株,以 0.3 M 蔗糖培養基預培養 5 天,切取 2 - 2.5 mm 莖頂,以冷凍保護劑 LS 處理 120 min, PVS2 處理 120 min,存活率可達 79.2 - 91.7%。春蘭與蝴蝶蘭 大白花品種'V3'經蔗糖預培養,分析莖頂水分生理與內含物變化,隨著預培養蔗 糖濃度與天數增加,莖頂累積可溶性醣類與可溶性蛋白,改變滲透潛勢,誘導莖 頂耐旱性與抗凍性,提升超低溫冷凍保存之存活率。苦瓜`F-948'花粉以 10%蔗糖濃度之 B&K 培養基萌發情況最佳,利用矽 膠乾燥劑進行脫水,測試冷凍保存前後花粉離體萌發率,經冷凍保存後,乾燥處 理 120 - 180 min,含水量介於 34.7 - 56.2%具有較高花粉萌發率 21.4 - 27.9%,以 乾燥處理 140 min 冷凍保存之花粉進行活體萌發測試,授粉結果、種子數、子代 植株外觀,證實花粉在液態氮處理後仍具有活性與授精能力。 本研究得知春蘭與蝴蝶蘭莖頂玻璃化法超低溫冷凍保存之最佳處理條件,與 苦瓜花粉乾燥法超低溫冷凍保存之最佳含水率範圍,期望能供為種原、種質超低 溫冷凍保存之參考。
URI: http://hdl.handle.net/11455/92157
文章公開時間: 10000-01-01
Appears in Collections:生命科學系所

文件中的檔案:

取得全文請前往華藝線上圖書館



Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.