Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/92631
標題: 八角蓮藥用植物增殖系統之建立
Establishment of multiplication protocol on medicinal plant Dysosma pleiantha (Hance) Woodson
作者: 林凱翔
Kai-Siang Lin
關鍵字: 八角蓮
Dysosma pleiantha (Hance) Woodson
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摘要: 本試驗目的在於探討藥用植物八角蓮利用微體繁殖的組織培養技術,建立其增殖系統,方法過程包含建立無菌培植體、癒傷組織誘導、芽體誘導、芽體增殖、發根與健化移植。癒傷組織之誘導以含有BA 0.2 mg.L-1+2,4-D 0.5 mg.L-1之培養基培養誘導效果最佳,誘導率達到88.2%;芽體誘導則以BA 1.0 mg.L-1+TDZ 0.1 mg.L-1之生長調節劑濃度培養基培養,芽體誘導分化率最高,達到33.2%;在芽體增殖方面,運用BA/NAA (0.1-2.0 mg.L-1/0.01-0.2 mg.L-1) 濃度以10:1比值之濃度梯度處理,發現以BA 2.0 mg.L-1+ NAA 0.2 mg.L-1濃度可以獲得最多為8.5個芽體;在發根試驗中,以1/2 MS+ IBA 0.5 mg.L-1 之培養基培養可以於第2週時誘導出根,誘導率約20%,於第4週時根誘導率達到100%;八角蓮在健化移植試驗中,使用發根6週以上之培植體,都可以順利移植,存活率均為100%。
The purpose of this test is therefore to examine the use of the tissue culture technology called micropropagation for reproducing the medicinal plant Dysosma and building its multiplication system, which involves methods and processes including preparation of sterile explants, blastemal induction, multiplication, rooting, hardening and transplanting. For the induction of wound healing tissues, placing the petioles of the plant in a culture medium that contained BA 0.2mg.L-1+2,4-D 0.5 mg.L-1 produced the best inductive effect, with an induction rate up to 88.2%. For blastemal induction, incubation using a growth regulator that contained BA 1.0 mg.L-1+TDZ 0.1 mg.L-1 achieved the highest induced blastemal fractionation rate, up to 33.2%. For blastemal multiplication, it was found that treating with BA/NAA in a concentration gradient of 10:1 (0.1-2.0 mg.L-1/0.01-0.2 mg.L-1) produced the largest number of blastemas, with an average of 8.5. In the rooting test, incubation using a culture medium that contained 1/2 MS+ IBA 0.5 mg.L-1 was able to induce rooting in the second week with an induction rate of about 20%, which increased to 100% in the fourth week. In the hardening and transplanting test, explants that had grown roots for at least six weeks were used and all of them, could be successfully transplanted and had a survival rate of 100%.
URI: http://hdl.handle.net/11455/92631
其他識別: U0005-2701201523332800
文章公開時間: 10000-01-01
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