Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/92928
標題: A hybrid statistical and genetic network approaches to analyze mouse brain microarray dataset
基於統計及基因網路混合式分析法於小鼠腦部微陣列基因數據之研究與應用
作者: 李佩容
Pei-Jung Lee
關鍵字: microarray
trace fear conditioning
hippocampus
paired-samples t-test
feature selection
微陣列基因晶片
痕跡恐懼實驗
海馬體
成對樣本T檢定
特徵選取
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摘要: Brain and spinal cord constitute the central nervous system, and central nervous system relies on complex linkages to deal with the transmission of the mysterious information. Among them, the brain's hippocampus is responsible for storing spatial memory, long-term memory and memory Integration. The calcium channel causes different intracellular biochemical reactions, including gene or hormones expression, nerve conduction and secretion. Previously it has been demonstrated that hippocampus will lost its main function while the Cav3.2 calcium channel knocked out. The atypical structures and functions of the Cav3.2 T-type calcium channel might causes Alzheimer's disease. Nowadays, gene targeting has been popularly used to study organism genetic diseases by knocking out or knocking in, specific mutations of interest to a variety of models. In this thesis, there are 39,200 gene expressions from microarray datasets of mouse brain. We used paired-samples t-test and feature selection as an analysis tool to search for target genes in the database. By these analysis methods, the selected genes will indicate its unique reference sequences, pathways and variations. This thesis is intent to figure out whether there are any differences of hippocampus gene expression through trace fear conditioning and Cav3.2 T-type calcium channel knocking out experiments. Furthermore, it will provides an advantageous direction for biological or medical studies of the filtered target genes of animal models.
人類大腦和脊髓所構成的中樞神經系統為最複雜且完整的,而中樞神經系統與周圍神經系統所傳遞的信息確保了生物體各個器官之運作、並將體內機能和體外活動作統整和協調。其中,位於大腦邊緣系統中的海馬體擔當著儲存空間定位、長短期記憶轉換、及記憶整合之任務。當海馬體受損時,會出現方向知覺喪失及記憶衰退等症狀。生物體內的鈣離子通道的化學本質為蛋白質,是一載體,調控著細胞內鈣濃度的升降及許多不同的細胞生化功能,如神經傳導、肌肉收縮、分泌作用、細胞移動、細胞增生、基因表現及細胞死亡。目前已有研究指出當海馬體剔除Cav3.2鈣離子通道後,會失去其針對記憶掌控之主要功能,可能導致阿茲海默症等相關病變。 現今醫療科學已廣泛的運用標靶基因晶片剔除及篩選過程,針對突變細胞進行基因分析,以區分未知基因並鎖定受藥物刺激的細胞進行觀察,以探討生物遺傳性疾病。本研究運用成對樣本T檢定做檢測及特徵選取做海量數據探勘,自小鼠腦內39,200筆微陣列基因晶片數據中挖掘與記憶相關之基因,並區別出經痕跡恐懼實驗前後、剔除鈣離子通道與否之小鼠基因反應,萃取並搜尋Hmga2、Enpp6、Spire1、Pzp及Flvcr2五筆基因之途徑及可能性疾病,佐與腦部病變導致記憶退化相關之文獻為證。本研究所使用之研究方法,將可提供生物學或醫療科學一有利的快速篩選參考方法。
URI: http://hdl.handle.net/11455/92928
其他識別: U0005-0806201523220300
文章公開時間: 10000-01-01
Appears in Collections:資訊管理學系

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