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|標題:||Construction of Automatic Information System Based on Microarray Data of Scopolamine-Treated Rat in Hippocampus|
Learning and Memory
Ingenuity Pathway Analysis
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|摘要:||Scopolamine is one of the competitive antagonist. It is the secondary metabolites from Datura. Scopolamine is being used to treat motive sickness. The side effects of this drug including dry mouth, muscle weakness, etc. People usually don't realize that functions of learning and memory may be affected by overdosing scopolamine.
Hippocampus is an important component of the brain. It associate with the functions of learning and memory. Short-term memory will be consolidated into long-term memory by hippocampus. New memory cannot be created by brain when hippocampus damaged.
In this study, microarray data of gene expression from scopolamine-treated rat is analyzed by statistical methods which include T-test and Linear Regression. An information system was created to analyze microarray data automatically. Six target genes, PRSS22, ETF1, TCN1, PRKAG1, MUTYH and EDN3, were selected from 9,600 genes on the array. A genetic pathway was created and target genes were studied by Ingenuity Pathway Analysis software. Finally, these genes analyzed from IPA, ETF1, TCN1, PRKAG1 and EDN3 are associated with the functions of learning and memory in hippocampus.|
東莨菪鹼（Scopolamine）是一種從曼陀羅花中提煉的藥物，作為一種乙醯膽鹼的拮抗劑使用，目前主要被用來治療動暈症狀，它能夠阻斷運動神經的傳導，對於暈車藥來說效果相當不錯。然而當東莨菪鹼藥物的劑量加大，其對於影響腦部學習與記憶功能的副作用會增加。 海馬迴（Hippocampus）是位於大腦顳葉中的一個區塊，主要負責將短期記憶組織後作為長期記憶來儲存，當海馬迴受損後便無法在增加新的記憶，故本論文旨在探討東莨菪鹼藥物對於海馬迴中的基因，是否會造成學習與記憶功能的障礙。 本論文使用大鼠服用東莨菪鹼藥物實驗的海馬迴微陣列晶片資料樣本，其中包含9600組基因表現量，使用T檢定來驗證假說，確定實驗前後大鼠海馬迴的基因表現量具有顯著差異，使用迴歸分析並計算殘差值，利用標準化殘差方法篩選出6個標靶基因，並利用IPA軟體來分析標靶基因之間的關係以及建立途徑網路，加以探討這些標靶基因對於學習與記憶功能的關聯性與影響。最後建置Matlab系統自動化分析，增加同類型資料研究效率。本論文篩選出PRSS22、ETF1、TCN1、PRKAG1、MUTYH以及EDN3共六個標靶基因，而其中ETF1、TCN1、PRKAG1與EDN3確實與腦部的學習與記憶功能有關聯，未來經過生物實驗驗證後，這些標靶基因便能作為生醫領域相關研究的依據，以提升對於學習記憶等相關功能的研究效率與成果。
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