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標題: Expression of MAGE-A in canine testicular, oral and round cell tumors
MAGE-A 蛋白在犬睪丸腫瘤、口腔腫瘤及圓形細胞腫瘤之表現
作者: Yi-Sheng Chang
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摘要: 黑色素瘤相關抗原-A (melanoma associated antigen-A, MAGE-A)是癌症/睪丸抗原(cancer/testis antigen)的一種。這些抗原只會表現在正常的原始生殖細胞及多種腫瘤細胞中。人類的 MAGE-A 基因共有 12 種亞型,分別為 MAGE-A1 到MAGE-A12。所有的 MAGE-A 都有一段高度相似的氨基酸序列,其大小約為 200個氨基酸。本研究室先前已經製備出鼠抗犬 18-kDa MAGE-A 片段之多株抗體,並且以免疫組織化學染色法來偵測犬正常組織中 18-kDa MAGE-A 的表現。在所有受測的正常組織中只有睪丸中的原始生殖細胞呈現陽性。本實驗進一步使用此抗體以免疫組織化學染色法偵測犬的睪丸腫瘤,口腔腫瘤以及圓形細胞瘤中 18-kDa MAGE-A 的表現。睪丸腫瘤包括 10 個精母細胞瘤,10 個賽托利細胞瘤及 10個間質細胞瘤;口腔腫瘤包括 17 個黑色素瘤,10 個鱗狀上皮細胞瘤,6 個纖維肉瘤及 12 個齒齦瘤;圓形細胞包括 12 個組織球相關疾病,11 個多中心型淋巴瘤,20 個肥大細胞瘤,10 個漿細胞瘤及 10 個傳染性花柳性腫瘤。結果顯示 18-kDa MAGE-A 在睪丸腫瘤中有 33% (10/30)的陽性率,在口腔腫瘤中有 24%(11/45)的陽性率,而在圓形細胞瘤中則有 33% (21/53)的陽性率。在這些腫瘤中,賽托利細胞瘤、間質細胞瘤、齒齦瘤及傳染性花柳性腫瘤皆無 18-kDa MAGE-A的表現,而精母細胞瘤(100%, 10/10)、淋巴瘤(54.5%, 6/11)及肥大細胞瘤(55%,11/20)則有明顯的陽性表現。經由 Fisher's exact test 的分析,18-kDa MAGE-A 在口腔黑色素瘤、淋巴瘤及肥大細胞瘤中的表現與否在不同的臨床分期或組織學分級之間並無顯著差異。由於 18-kDa MAGE-A 只表現在犬正常的原始生殖細胞與多種腫瘤中,此蛋白或許可做為腫瘤的生物標的蛋白。
Melanoma associated antigen-A (MAGE-A) is one superfamily of the cancer/testis antigens which is only expressed in normal primary germ cells and various types of cancers. There are 12 subtypes of MAGE-A in human, from MAGE-A1 to MAGE-A12. All of MAGE-A share a homology domain consisting of about 200 amino acids. A polyclonal mouse anti-dog 18-kDa MAGE-A antibody was produced in our lab. This antibody was applied to detect 18-kDa MAGE-A in normal canine tissues by immunohistochemical staining. Results revealed that all the tested normal canine tissues were negative except testicular germ cells. The expression of 18-kDa MAGE-A in canine testicular, oral and round cell tumors was further investigated by immunohistochemical staining with the same antibody. Testicular tumors included 10 seminomas, 10 Sertoli cell tumors and 10 Leydig cell tumors. Oral tumors comprised of 17 melanomas, 10 squamous cell carcinomas, 6 fibrosarcomas and 12 epulides. Round cell tumors consisted of 12 histiocytic diseases, 11 multicentric lymphomas, 20 mast cell tumors, 10 plasmacytomas, 10 transmissible venereal tumors. The positive immunolabelling of 18-kDa MAGE-A were 33% (10/30) in testicular tumor, 24% (11/45) in oral tumors and 33% (21/63) in round cell tumors. Among these tumors, negative immunostaining were showed in Sertoli cell tumors, Leydig cell tumors, epulides and transmissible venereal tumors, but significant immunoreactivity was observed in seminomas (100%, 10/10), multicentric lymphomas (54.5%, 6/11) and mast cell tumors (55%, 11/20). There was no statistical difference between the expression of 18-kDa MAGE-A and tumor stages or grades of oral melanoma, multicentric lymphoma and mast cell tumor in Fisher's exact test. Based on its restrictive expression in normal canine germ cells and various types of neoplasm, 18-kDa MAGE-A has the potential to be used as a biomarker for tumors.
其他識別: U0005-0605201514512300
文章公開時間: 10000-01-01
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